H2020Индивидуална стипендия2021–2023

TICKITS · SFTS virus-tick cell interactions in a vector system.

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-06-01 → 2023-05-31
Финансиране от ЕС: 224 934 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Взаимодействията между вируса SFTS и клетките на кърлежите се анализират чрез проучване на протеините и гените им. Това помага за по-доброто разбиране на механизмите, по които вирусите се разпространяват чрез кърлежи, което е важно за контрола на инфекциите при хората.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

SFTS virus-tick cell interactions in a vector system.

Ticks serve as vectors for arboviruses in various regions worldwide. The rising prevalence and emergence of tick-borne arbovirus infections among human populations emphasize the importance of implementing vector control strategies. However, our understanding of the cellular-level interactions between arboviruses and ticks remains limited, with a significant gap in knowledge regarding negative strand RNA arboviruses. Studying tick-arbovirus interactions presents significant challenges due to the lack of fundamental genomic data for non-model arthropods, such as Rhipicephalus microplus ticks, the natural vector of the emergent Dabie bandavirus (Bunyavirales; Phenuiviridae) previously known as severe fever with thrombocytopenia syndrome virus. To overcome these limitations, the TICKITS (TICK cell Interactions with SFTSV) project aims to employ a system virology approach to provide an unprecedented understanding of SFTSV interactions with vector tick cells. The findings will be of high relevance for tick-borne bunyaviruses. The novel and innovative aspect of our project is the combination of vector tick cells with cutting edge transcriptomics and proteomics techniques to study virus-host interactions in this non-model organism. First, it will provide (1) novel insights on gene and proteome regulation during SFTSV infection of vector tick cells, highlighting both pro- and anti-viral factors, (2) allow us to identify the specific viral-host protein interactions which may be involved in re-wiring cellular pathways during infection and (3) take advantage of the silencing technology to assess the function of a selected set of genes during SFTSV infection of tick cells (for example immune genes or viral protein interactors). This integrative perspective of the host response will generate valuable outcome and new knowledge on tick-borne virus infection. The generation of novel genomic and proteome data will be reinforced by the first description of intracellular tick-bunyavirus protein interactions. Though my research is of fundamental nature, these findings could be translated into proof-of-concept work on manipulation of cellular pathway of the tick vector to control viral infections in the future.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Among hematophagous arthropods ticks transmit a large variety of pathogens with public health and veterinary importance. Tick transmit several emergent and re-emergent arboviruses across the world. In 2010, scientists discovered severe fever with thrombocytopenia syndrome virus (SFTSV), an emergent Banyangvirus (Phenuivirus) transmitted by ticks in Asian countries. SFTSV infection causes severe symptoms and can lead to the death in infected individuals. Novel vector-based control strategies require a more detailed understanding of the vector-virus interaction and the vector response to infection. Ripicephalius microplus ticks are vectors of SFTSV and as for many non-model arthropods we are missing basic genomic data, which makes tick-arbovirus interaction studies difficult. To overcome these limitations, the TICKITS (TICK cell Interactions with SFTSV) project aims to develop an innovative approach coupling transcriptomic and proteomics data from R. microplus cells during infection. This systematic approach will characterize SFTSV infections in their natural tick vector and will generate information on changes in vector cells both at transcriptome and proteome level. This integrative view of the host response will give us insights on targets that can be investigated using gene silencing approaches to determine their impact on viral infection. I choose to work with the Kohl and Brennan laboratories at the MRC-University of Glasgow Centre for Virus Research (CVR) in Glasgow, UK. Both laboratories excel in tick/arthropod biology and arbovirus infection studies and have all the tools necessary to carry out this work. By adding my molecular and omics expertise we aim to develop novel angles in tick research. Moreover, the data generated will provide a solid base for other projects to build on and develop comparative projects across species.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITY OF GLASGOW · GlasgowКоординаторОбединеното кралство