H2020Индивидуална стипендия2021–2023

TVISTOFF · Tick-Virus Interactions Shape persistence and Transmission OF Flavivirus pathogen in tick vector

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-09-01 → 2023-08-31
Финансиране от ЕС: 155 364 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Взаимодействието между протеините на вируса на кърлежния енцефалит и нервната система на кърлежите определя как патогенът оцелява и се предава. Това помага да се разбере разпространението на заболяването, което причинява невродегенерация и е риск за здравето в Европа.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Tick-Virus Interactions Shape persistence and Transmission OF Flavivirus pathogen in tick vector

The project entitled “Tick-Virus Interactions Shape persistence and Transmission OF Flavivirus pathogen in tick vector” is concerned with the role of structural protein E of the tick-borne encephalitis virus and tick neurosecretion system in the virus persistence and transmission in tick vector. The research goals suggested the development of novel tools that would be instrumental in studying how the virus persists in ticks and how ticks harbor the virus. Such goals are important due to the lack of information on virus-tick interactions. Moreover, tick-borne encephalitis poses a health risk not only in Slovakia but also in several other European countries. Despite an available and effective vaccination, cases of tick-borne encephalitis have been on the rise and include severe cases with neurodegeneration and up to 2% fatality rate. So far, most of the research on tick-borne encephalitis has involved a virus-mouse model, whereas virus-tick models have been understudied. Needless to say, it is true that the manipulation of a genetically modified live virus and experimentally infected ticks might be challenging as it is interdisciplinary and constitutes a biohazard, for which many institutions are not equipped. Society is often interested in fundamental questions regarding virus transmission in ticks, such as speed of transmission, and what tick species participate in it and how. Virus-tick interactions have ever been an essential part of virus circulation in nature about which we have had only fragmented information. Therefore, the fellow took advantage of available facilities and combined various interdisciplinary approaches based on his skills and knowledge to identify aspects of interactions between viruses and ticks in a comprehensive way for society. Objectives of this MSCA have been to (i) construct and characterize a chimeric virus with mutated E protein and a virus carrying a fluorescent reporter gene; (ii) set up and optimize an artificial membrane system for tick feeding (without an animal) to study the speed of virus transfer; (iii) characterize tick biogenic amines, neuropeptide and their receptors in response to tick-borne encephalitis virus. A parallel aim of this MSCA is to set up an innovative research program and to advance the development of the fellow towards independent research leadership.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

In Europe, tick-borne encephalitis virus (TBEV) causes tick-borne encephalitis, the most important viral life-threatening disease transmitted by ticks. Interactions among the virus, tick and host are inseparable and paramount for a successful circulation of TBEV in an enzootic cycle. Nevertheless, research in this area has predominantly focused on TBEV pathogenesis in vertebrate hosts. Limited knowledge on virus-tick interactions points out to lack of systematic studies and warrants research implementing novel virus-tick models. Therefore, the goal of this fellowship is to investigate the roles of molecular factors of TBEV virulence and tick neurosecretory compounds in adaptation and transmission of TBEV. The innovative concept involves a development of fluorescently labelled TBEV mutants by reverse genetics, implementation of host-free artificial membrane tick feeding system, and characterising components of tick neuroendocrine systems. The interdisciplinary approach of interconnecting the applicant's unique skills in tick physiology and bacterial tick-borne pathogens, expertise of the host group in arbovirology and capacity of partner group in physiology of tick neuroendocrine systems will be instrumental in widening knowledge on virus-tick interactions. Developed tools and obtained results will provide invaluable foundation for research of other neglected tick-borne viruses. Taking together, this fellowship will enable the applicant to reintegrate into the host organisation in his home country, to carry out innovative research, and ultimately to reach professional maturity as an independent investigator.

Оригинален текст от CORDIS (на английски).

Участници

BIOMEDICINSKE CENTRUM SLOVENSKEJ AKADEMIE VIED, VEREJNA VYSKUMNA INSTITUCIA · BratislavaКоординаторСловакия