H2020Индивидуална стипендия2021–2024

PERSIST · Antibiotic persisters during infection: a tail of intestinal dominion

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-11-01 → 2024-10-31
Финансиране от ЕС: 257 620 €
Участници: 2
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Бактериалните „персистери“ са клетки, които временно спират да растат, за да оцелеят след прием на антибиотици. Разбирането на техните функции помага за разработването на стратегии за справяне с инфекциите, които често се връщат след лечение.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Antibiotic persisters during infection: a tail of intestinal dominion

Persisters are transiently non-growing bacteria cells that are able to evade antibiotic treatment and immune response. These pathogenic bacterial persisters, are responsible for the frequent relapses of apparently successfully treated infections (persistent infections) and the spread of mobile genetic elements that encode antibiotic-resistance genes. As a result, persisters contribute to the global threat of antibiotic treatment failure and, therefore, to patient morbidity and mortality and increased medical costs. Novel strategies to eradicate such persistent infections and, in general, the failure of antibiotic treatment that are based on fundamental research address a problem of high clinical, scientific and societal urgency. The overall objective of the work carried out during this fellowship was to study the physiology of persisters in order to aid in the development of experimentally validated strategies to eradicate them.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Persisters are transiently non-growing bacteria cells that evade antibiotic treatment and immune response. Persisters have been associated with antibiotic treatment failure and the spread of antibiotic-resistance (AMR) through mobile genetic elements. Consequently, persisters contribute significantly to the morbidity and mortality of bacterial infections, and increased medical costs. Although it is known that many pathogenic bacteria are able to form persisters, the occurrence of persistence among commensal bacteria is yet unexplored. This project aims to identify and exploit commensal persisters able to antagonise and displace pathogenic persisters, offering opportunities for the development of innovative treatment options to arrest both the relapse of persistent infections and the horizontal transfer of AMR. To this effect, I will use a combination of cutting-edge omics-based tools, in vivo murine models and the well-established and relevant Salmonella enterica serovar Typhimurium enteric model pathogen. After identifying commensal species forming persisters (WP1), I will assess the ability of these persisters to compete with Salmonella persisters during infection (WP2) and to arrest in vivo horizontal gene transfer from Salmonella persisters to intestinal microbiota (WP3). My goal is to become a leading academic scientist in the intertwining fields of antibiotic-resistance and antibiotic-persistence, with emphasis on the involvement of persister cells in the maintenance and spread of mobile genetic elements encoding AMR. The state-of-the-art computational and experimental training at the pioneering groups of in vivo persister biology at Harvard Medical School (HMS) in USA and of genome spatial organization at the Institut Pasteur (IP) in France will be instrumental towards achieving my goal. Apart from empowering my career track, this fellowship will foster future collaborations between HMS and IP, and promote transfer of knowledge in Europe.

Оригинален текст от CORDIS (на английски).

Участници

INSTITUT PASTEUR · ParisКоординаторФранция
PRESIDENT AND FELLOWS OF HARVARD COLLEGE · CambridgeСъединени щати