HEИндивидуална стипендия2022–2026

Si-ORHIGENS · Unravelling the Silicification prOcess in RHIzaria through GENetics and Skeletal growth

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2022-11-01 → 2026-08-31
Финансиране от ЕС: 300 442 €
Участници: 2
Схема: HORIZON-TMA-MSCA-PF-GF

Линиите свързват координатора с партньорите.

Накратко на български

Ризариите (едноклетъчни морски организми) и начинът, по който изграждат своите силициеви скелети, се анализират чрез генетика и биоинформатика. Това помага да се разбере по-добре ролята им в глобалния цикъл на силиция и въглерода в океана.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Unravelling the Silicification prOcess in RHIzaria through GENetics and Skeletal growth

Silicon (Si) is a critical nutrient in the global ocean, essential for the growth of a wide range of organisms, including microalgae, sponges, and rhizarians. Silicifying Rhizaria were among the first protists to inhabit the ocean and, alongside sponges, dominated biogenic silica production during the Cambrian era. Despite their significance, these organisms remain poorly understood as key components of marine ecosystems. In today’s ocean, diatoms are widely recognized as the primary contributors to the Si cycle within the planktonic realm. However, recent research has uncovered that Rhizaria exhibit unexpectedly high individual Si production rates and biogenic silica content, indicating that their role in marine Si and carbon cycles may have been underestimated. This project seeks to elucidate the process of silicification in Rhizaria and to identify the factors driving variability in silicic acid uptake. To achieve this, the work is divided into three integrated components. The first involves analyzing global transcriptomic data and individual transcriptomes of isolated Rhizaria to investigate the diversity of Si transporters. The second focuses on examining silica deposition using fluorescence markers across a wide range of Rhizaria. The third component quantifies gene expression during silicification, employing primers developed from the first component. This interdisciplinary project leverages bioinformatics, molecular biology, and physiology, presenting a unified approach to studying plankton. Its outcomes will significantly enhance our understanding of Si transport in Rhizaria and their role in the ocean's biogeochemical Si cycle.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Silicon (Si) is a major nutrient in the global ocean, required for growth by a variety of distantly related organisms, including microalgae, sponges and rhizarians. Silicifying Rhizaria were among the first protists to inhabit the ocean and, together with sponges, dominated the biogenic silica production during the Cambrian era. These organisms became an important, though poorly understood, component of marine ecosystems. In the contemporary ocean, diatoms are thought to dominate the planktonic realm and to be the main contributor to the Si cycle. However, my recent work has revealed that Rhizaria have unexpectedly high individual Si production rates and biogenic silica content, suggesting that their contribution to marine Si and carbon cycles may have been underestimated. This project aims to elucidate the process of silicification in Rhizaria and determine sources of variability in silicic acid uptake of these protists, through three tailored work packages (WPs). The first WP, consists of the analyse of transcriptomic data at a global scale as well as individual transcriptomes of isolated Rhizaria to assess the diversity of Si transporters. Thereafter, the examination of silica deposition, using a fluorescence compound, in a broad range of Rhizaria and varying ambient silicic acid concentrations. The final WP, will be the quantification of gene expression during silicification processes using primers designed within WP1. This highly interdisciplinary project combines bioinformatics, molecular biology and physiology, leading to a more unified approach to plankton research. The outcomes of this project will improve our general understanding of Si transport in Rhizaria as well as our understanding of the biogeochemical cycling of Si in the ocean. This work will also constitute a major advance in my career, as I acquire skills in new research methods beyond my current capacity, and build a unique skillset that will set me ahead of the current rhizarian research scope.

Оригинален текст от CORDIS (на английски).

Участници

SORBONNE UNIVERSITE · ParisКоординаторФранция
MONTEREY BAY AQUARIUM RESEARCH INSTITUTE · Moss LandingСъединени щати