HEИндивидуална стипендия2022–2024

PSEUDOPIN · PSEUDOPIN

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2022-05-01 → 2024-04-30
Финансиране от ЕС: 214 934 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Псевдоуридинът е модификация на РНК, която променя стабилността и структурата на молекулите в растенията при атака от патогени. Разбирането на този процес помага за създаването на по-устойчиви сортове култури.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

PSEUDOPIN

RNA contains hundreds of covalent modifications, primarily found in non-coding RNAs such as transfer RNAs (tRNAs) and small nuclear RNAs (snRNAs), though these modifications are also present in messenger RNAs (mRNAs) and small RNAs (sRNAs). These RNA modifications are crucial for genetic regulation, as they influence fundamental RNA properties including stability, structure, and interactions with proteins and other RNAs. This, in turn, regulates essential cellular processes such as splicing and translation. The project titled "Unraveling the Impact of Pseudouridine RNA Modification upon Plant Immune Activation" (PSEUDOPIN) investigates the role of the RNA modification pseudouridine in plants. Specifically, it explores how changes in the position and abundance of pseudouridine contribute to the genetic reprogramming triggered by pathogen detection. By studying these dynamics, the project aims to elucidate how pseudouridine modification impacts plant immune responses and its broader implications for plant resilience. Research in this area is of paramount importance due to the current lack of fundamental understanding regarding the role of pseudouridine RNA modification in plant RNAs. There is even less information (nothing) about its function as a key element in regulating gene expression during pathogenicity. The knowledge generated from this project is highly valuable not only to the plant and RNA research communities but also to breeding and seed companies. This understanding could significantly impact the development of resilient crop varieties and advance our comprehension of RNA modifications in plant biology. The project aims to address two critical questions: (i) uncover the role of RNA pseudouridylation in regulating genetic information in plants, and (ii) analyze how this RNA modification influences plant immunity by impacting key cellular processes such as mRNA splicing and/or translation. To address these open questions, the objectives of this Marie Skłodowska Curie Action (MSCA) have been: 1) Identify PUS5-dependent Ψ-sites upon immune receptor activation. 2) Investigate the molecular processes, e.g. splicing or translation, affected by PUS5-mediated pseudouridylation. 3) Perform functional analysis of selected PUS5-dependent Ψ sites, if such sites can be identified. 4) Analyse the biological consequences of PUS5-dependent pseudouridylation for the immune response.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Pseudouridine () is abundant in major classes of non-coding RNA (ncRNA), including ribosomal RNA, transfer RNA, and spliceosomal RNAs. The Watson-Crick base pairing potential of is identical to that of uridine, but may stabilize RNA structures and engage in RNA interactions distinct from those of uridine, probably explaining its requirement for cellular viability. In addition to the many constitutive -sites in major ncRNAs, recent reports have uncovered induced by specific stimuli or present in specific cell types, in rare cases with demonstrated importance for cellular translation and splicing patterns. Nonetheless, the full extent of occurrence and regulatory consequences of induced pseudouridylation remain unknown, highlighting the outstanding potential for discovery on this topic. PSEUDOPIN builds on my observation that the gene encoding PseudoUridine Synthase PUS5 in the plant Arabidopsis thaliana exhibits mRNA isoform switching upon immune receptor activation: in growing seedlings, PUS5 encodes an organellar enzyme, but immune receptor activation induces transcription initiation at a downstream site to exclude the organellar target peptide from the PUS5 protein, thus giving rise to a nucleo-cytoplasmic enzyme. Two questions are being investigated here: (i) can we identify PUS5-dependent -sites upon immune receptor activation, and (ii) can we define functions of selected induced -sites in the growth-to-defense genetic reprogramming? PSEUDOPIN uses state-of-the-art sequencing methods, including direct RNA sequencing technology, to systematically map in major RNA classes after immune receptor stimulation. It also lays out strategies to select and study particularly interesting cases. The topic remains completely uninvestigated in plants, so the project promises to break open a new research field within plant gene expression, and thereby provide material to start an independent research career following successful completion of the fellowship.

Оригинален текст от CORDIS (на английски).

Участници

KOBENHAVNS UNIVERSITET · KOBENHAVNКоординаторДания