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HEИндивидуална стипендия2022–2024

SquidVibrio · Characterization of multi-species bacterial interactions underlying the beneficial symbiosis of a Mediterranean squid.

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период
2022-09-01 → 2024-08-31
Финансиране от ЕС
211 755 €
Участници
2
Схема
HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Взаимоотношенията между средиземноморския калмар Sepiola affinis и различните видове бактерии Vibrio в неговия светещ орган се анализират чрез нова експериментална система. Това помага за разбирането на сложните взаимодействия между микробите и здравето на животните.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Characterization of multi-species bacterial interactions underlying the beneficial symbiosis of a Mediterranean squid.

Beneficial host-microbe interactions play a crucial role in maintaining animal health, yet their complexity makes them challenging to study. Invertebrate models for mutualism, however, offer valuable insights into the function of these associations. One such model is the squid-vibrio symbiosis that examines the partnership that occurs within a symbiotic light organ. The symbiosis has been studied extensively in the USA focusing on the Hawaiian bobtail squid Euprymna scolopes and it’s bioluminescent bacterial symbiont Vibrio fischeri. The SquidVibrio Project aims to expand studies of the squid-vibrio symbiosis into Europe by studying Sepiola affinis, a species of Mediterranean squid. Intriguingly, S. affinis is colonized by multiple Vibrio spp., allowing for examination of how interspecies bacterial interactions persist within the symbiosis. The overarching aim of the project is to establish the first experimental system to study the squid-vibrio symbiosis within Europe. Furthermore, the project will serve to (1) establish a reliable husbandry system for S. affinis, (2) examine multiple Vibrio species within the squid’s symbiotic light organ and (3) characterize small molecule patterns within the light organ. The experimental framework outlined here demonstrates how the SquidVibrio Project is positioned to introduce an exciting new model organism to Europe while making significant contributions to the fields of symbiosis and marine biology.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Animals often use associations with microbes to preserve their health. These mutualisms are maintained by host-microbe communication and though interactions between bacterial symbionts. The squid-vibrio symbiosis has been used for over 30 years to study beneficial host-microbe associations. However, nearly all studies of the symbiosis have used Euprymna scolopes and its only symbiont, Vibrio fischeri, which precludes examination of how multiple microbial species affect symbiosis. Using the squid Sepiola affinis, and its two symbionts, V. fischeri and Vibrio logei, the SquidVibrio project proposes to examine the effects of multiple microbial species within a mutualism. The project is organized into three objectives. 1) Implement a husbandry facility for S. affinis. 2) Quantify the symbiont populations within the S. affinis light organ over seasons and over maturation. 3) Identify how the symbiont populations alter metabolites within the light organ. First, a husbandry facility will be established at the Banyuls-sur-Mer Oceanographic Observatory that will allow for the maintenance of wild S. affinis and for rearing of the squid. Second, metagenomics will be used to estimate the proportions of V. fischeri and V. logei within the light organ. Subsequently, light organs of field-caught and reared squid will be imaged using confocal microscopy to identify if the Vibrio spp. inhabit mixed or distinct niches. The third objective will use LC-MS and mass spectrometry imaging to identify metabolites that are altered by each Vibrio spp. and how localization patterns of these molecules are affected. These methods will allow for the two-way transfer of knowledge, with the researcher providing squid husbandry techniques and the host institution providing genomics and metabolomics. The SquidVibrio project will establish S. affinis as a model organism in Europe that can provide insights into how symbionts interact both with each other and their host to underly a mutualism.

Оригинален текст от CORDIS (на английски).

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Данни: CORDIS, © Европейски съюз