HEИндивидуална стипендия2023–2025

NanoEXOS · Towards a Mechanistic Understanding of Nanoparticle Interactions with Exosome Secretion

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-09-01 → 2025-08-31
Финансиране от ЕС: 199 694 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Наночастиците и начинът, по който те влияят върху създаването на екзозоми – малки частици, чрез които клетките си комуникират. Това помага за разработването на по-безопасни наноматериали и нови методи за диагностика и лечение на заболявания.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Towards a Mechanistic Understanding of Nanoparticle Interactions with Exosome Secretion

CONTEXT AND OBJECTIVES NanoEXOS was conceived to address a fundamental and still unresolved challenge in bionanoscience: understanding how engineered nanoparticles (NPs) interact with the cellular machinery that produces exosomes. Exosomes are nanosized cell-released particles enriched in biologically active molecules such as extracellular RNAs and proteins. They act as key mediators of intercellular communication and are increasingly recognised for their central roles in both physiological regulation and disease development. The latter includes cancer progression, immune alterations, and the pathogenesis of liver, metabolic, cardiovascular, infectious, and neurodegenerative disorders. Despite their growing biomedical relevance, the precise mechanisms through which exogenous NPs alter or influence exosome formation remain poorly understood. This knowledge gap limits the capacity to design safe nanotechnologies and hinders the development of therapeutic and diagnostic strategies that might harness the potential of these extracellular nanostructures. By focusing on the interaction between NPs and exosomal pathways, NanoEXOS aimed to establish a novel mechanistic framework for understanding, predicting, and ultimately controlling NP–induced cellular responses. PATHWAY TO IMPACT The expected impacts of NanoEXOS are scientific, technological, and societal. Scientifically, the project contributes to a deeper understanding of bio-nano interactions at the cellular level, an essential step towards the creation of predictive models of NP safety and functionality. Technologically, the knowledge gained through NanoEXOS can be used to design next-generation nanomaterials, enabling safer biomedical applications and supporting the emergence of exosome-inspired nanomedicines. Strategically, the project contributes to strengthening Europe’s position in cutting-edge nanomedicine research, with an emphasis on healthcare innovation. The potential scale of impact is significant: safer and more advanced nanomedicine will enable progress towards personalised healthcare and reduced health risks, while biomedical innovations based on exosome biology could help address major health challenges such as the treatment of cancer, neurodegeneration, and infectious diseases. In this sense, NanoEXOS contributes both to advancing scientific frontiers and to creating pathways for tangible societal benefits.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

In recent decades, considerable progress has been made in bio-nano research towards a growing understanding of the interaction mechanisms between nanoparticles (NPs) and living systems. Yet, the impact that cell-internalised NPs have on exosome secretion remains largely unknown. Exosomes are ubiquitous cell-released vesicles seemingly involved in numerous biological processes, including regulation of cell-cell communication and disease progression. This enigmatic functional complexity is under the spotlight of a large scientific community in the fields of chemistry, biology and nanomedicine.Only recently, it has been reported that in vitro cellular uptake of platinum and iron oxide NP clusters (~ 100 nm in size) significantly increases the release of exosomes and alters their composition. However, it is unclear whether this evidence reflects a general behaviour or depends on the design of the NPs. A strong need thus arises to explore the mechanistic details of this interaction. Inspired by this challenge, NanoEXOS aims to develop a novel framework to obtain a better and more general understanding of how engineered NPs interfere with the regulatory processes of exosome formation and release. NP-cell studies will be undertaken in vitro to focus on the effects that NPs have on cell-specific exosome samples. Importantly, NanoEXOS will systematically investigate how this interaction depends on the properties of NPs (e.g. well-defined size, shape, surface modification), their biomolecular interactions and their cellular entry mechanism.Overall, NanoEXOS will contribute to expanding our knowledge of how exogenous NPs are processed by living systems. A thorough comprehension of how NPs access and alter key cellular machineries, such as exosome secretion, plays a pivotal role in the biological evaluation of these nanomaterials. This research question is crucial to establish safe and robust biological control over advanced NP-assisted diagnostic and therapeutic strategies.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN · DublinКоординаторИрландия