HEИндивидуална стипендия2023–2025

NIPMAP · Non-Invasive Predictive Modelling of Amphibian and Pathogen Diversity

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период
2023-03-01 → 2025-04-30
Финансиране от ЕС
188 590 €
Участници
3
Схема
HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Разнообразието на влечугите и патогените, като гъбички и вируси, се проследява чрез анализ на ДНК от проби вода и кожа. Това помага за по-бързото откриване на болести и планирането на опазването на видовете без директен контакт с животните.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Non-Invasive Predictive Modelling of Amphibian and Pathogen Diversity

Amphibians are among the most threatened animals on the planet, and populations are rapidly decreasing and even disappearing as a result of habitat loss, pollution, climate change, and infectious diseases. In particular, pathogens such as chytrid fungi and Ranaviruses have caused population declines across Europe. Conservation efforts require tools that are cost effective, rapid, and non-invasive so that fast monitoring of species and pathogens can be performed with minimal disturbance to the animals and habitat. The NIPMAP project (Non-Invasive Predictive Modelling of Amphibian and Pathogen Diversity) aimed to develop these tools by integrating molecular methods using DNA collected from amphibian habitats (e.g., pond water), generating maps, and creating open access data repositories. The goal was to create a field-to-conservation-practices ready system to detect, monitor and predict amphibians and pathogens in the environment without direct animal handling. Focussing on amphibian communities in Alpine habitats, the project combined field sampling with genetic analyses and predictive mapping. DNA from swabs collected from the skin of amphibians and filtered water samples was used to detect species and pathogens and subsequently to build models predicting their presence across diverse environments. These models inform conservation planning, even in areas with limited field access. To ensure transparency and reusability, data and analytical code are archived on open access platforms such as OpenBioMaps and GitHub, in formats that help stakeholders visualise and interpret results. NIPMAP worked closely with researchers, park managers, and citizen scientists, building bridges between science and society. Outreach efforts included public seminars and teaching activities to raise awareness on amphibian health and biodiversity. The project delivered a replicable, open framework for long-term monitoring of amphibian populations and emerging pathogens across Europe.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The current global biodiversity crisis requires rapid, inexpensive and reliable methods of detecting the main threats to species survival: pathogens, habitat change, and loss of genetic diversity1. Equally crucial are solutions for stemming biodiversity loss: we lack vital models that determine how these threats interact, which would help prioritise conservation efforts. Recently, CRI-CG has discovered for one species of frog over a relatively small geographic area that eDNA extracted from water samples captures more genetic diversity than analyses using tissue samples (UNESCO-funded project ACQUAVIVA). The same eDNA can be used to detect amphibian pathogens. NIPMAP will apply this knowledge in an innovative way by: i) optimizing this eDNA approach to estimate mtDNA diversity in four amphibian taxa (two caudates, two anurans) and pathogen diversity (principal pathogens: Bd, Bsal, Rv) across the eastern Italian Alps; ii) using these data to inform individual-based and spatially explicit simulations of host movements and pathogen transmission37; iii) integrating parameters from i-ii with skin microbiota and other health indices, as well as iv) environmental variables at each sample site, to generate correlative models (e.g. GLMMs and SDMs) and potential distribution maps to direct conservation management decisions. We will also pilot two innovative eDNA protocols: one to measure nuclear DNA diversity (to measure current gene flow and dispersal rates) and one to understand microbiota function using metatranscriptomics. These interdisciplinary tools will be integrated into a workflow that will be applicable to animal taxa anywhere on the planet.

Оригинален текст от CORDIS (на английски).

Участници

Връзки

Данни: CORDIS, © Европейски съюз