HEИндивидуална стипендия2023–2026

ImmunoZoneHubs · Immune Cell Death Zonation Regulated by Protein Hubs in Plants

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-09-01 → 2026-03-31
Финансиране от ЕС: 165 313 €
Участници: 3
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Растенията контролират локалното „самоубийство“ на заразените клетки, за да спрат инфекциите, без да увредят здравите тъкани. Разбирането на този механизъм помага за създаването на биологична устойчивост на културите, което намалява нуждата от химически пестициди.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Immune Cell Death Zonation Regulated by Protein Hubs in Plants

Global agriculture faces the dual challenge of a growing population and escalating threats from plant pathogens migrating due to climate change. Fungal and bacterial infections destroy up to 40% of global crop yields annually, resulting in billions of euros in losses and threatening food stability. Historically, the primary solution has been chemical pesticides. However, the European Green Deal and the "Farm to Fork" strategy aim to reduce pesticide use by 50% by 2030, necessitating a shift toward biological, "built-in" plant immunity. This project addressed a critical gap in understanding how plants manage Immunity-related Cell Death (ICD). When a plant recognizes a pathogen, it triggers a localized "suicide" of infected cells to halt the infection. For this to be effective, the plant must precisely control the death zone and ensure surrounding cells are "primed" to survive. Without strict control, runaway tissue damage can kill the entire plant. The project addressed the lack of knowledge regarding the molecular "boundaries" of this process and the protein machinery triggering it. The project pursued two primary objectives: Objective 1: Defining Spatial Boundaries and marker genes in Arabidopsis. We sought to resolve the spatiotemporal dynamics of the immune response by identifying "marker genes" that distinguish between cells undergoing suicide and healthy surrounding tissue. This provides the first high-resolution mapping of cellular boundaries that confine infection. Objective 2: Characterizing protease-containing protein hubs. We focused on the Metacaspase 1 (MC1) protease to decipher how signaling platforms (hubs) are assembled at the cell surface to transduce the detection of a pathogen into a coordinated cellular response.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Agriculture, which underpins the livelihoods of over 2.5 billion people, is nowadays confronted with an array of new threats including plant pests. This project tackles the urgent need to develop new, technology-based sustainable strategies to combat the constant agricultural losses and economic drain derived from plant pests, which have been worsened because of climate change. The understanding of the molecular and cellular mechanisms underlaying plant pathogen interaction and defence responses is not only a fundamental scientific challenge, but it is also relevant to practical applications. Despite decades of research on host-pathogen interactions, knowledge on fundamental aspects of the plant immune system is still missing. This proposal provides an excellent opportunity to help filling this gap of knowledge as it targets the question of how cells translate molecular pathogen recognition into immune cell death, and how dying cells and their neighbours communicate to tightly control this process and ensure the proper balance in between defence responses and plant growth and fitness. To address this question, I have designed a multidisciplinary and international project using Arabidopsis thaliana plant as a model system to study the molecular determinants of plant immune cell death zonation and the involvement of plant metacaspases, distant relatives of animal caspases, in cell death. A plethora of techniques including molecular and biochemistry techniques, plant phenotyping and engineering, FACS and cell biology microscopy techniques will be used for the proper execution of this project. In addition to generate high-impact knowledge in plant immunity that will ultimately impact on society, this proposal will provide me with the expertise, resources, training, and network to become extremely competitive in the field of molecular plant pathology, helping me to progress in my scientific career and to achieve my scientific goals.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE DE RECERCA EN AGRIGENOMICA CSIC-IRTA-UAB-UB · CERDANYOLA DEL VALLESКоординаторИспания
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS · ParisФранция
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV · MUNCHENГермания