HEИндивидуална стипендия2023–2025

SC-Foxy · Resolving temporal immune responses of Arabidopsis roots to infection by Fusarium oxysporum at the single-cell level

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-03-10 → 2025-03-09
Финансиране от ЕС: 189 687 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

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Накратко на български

Имунните реакции на корените на растението Arabidopsis при инфекция от гъбичката Fusarium oxysporum се наблюдават в реално време на ниво отделна клетка. Това помага да се разбере точно кога, къде и в кои тъкани се активира защитата на растението.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Resolving temporal immune responses of Arabidopsis roots to infection by Fusarium oxysporum at the single-cell level

While there is tremendous knowledge about the plant immune system, and how the plant defends itself against pathogens, we lack spatial resolution. Plant immune responses are typically recorded via transcriptomics (RNA-seq or qRT-PCR using pooled total RNA/DNA), in vitro assays (Calcium burst in cuvette, kinase phosphorylation in-gel, ROS-burst in cuvette), cell death assays in heterologous system (leaves with infiltrated effectors or pathogens) or with markers using leaves of transiently transformed and expressed proteins. In all cases, the spatial resolution is lost, and the infection is far from natural (the pathogen or its effectors are infiltrated in leaves, or physical damage is done by hand). To close this knowledge gap, we are using fluorescent markers for a wide array of plant immune pathways in live-imaging experiments. We allow Fusarium oxysporum, an important plant pathogen, to infect the plant by itself and in the tissue it naturally infects, without any infiltration, or dripping onto a certain tissue, by growing plant and pathogen in parallel in one sealed experimental dish. Once the fungus has infected the plants, we live-image to infection process and the immune responses of the plant via the different markers live and in real-time on a microscope. By doing so, we can map which immune responses are triggered at which time point, in which tissues, and in which cell types with cellular resolution, and while simultaneously imaging progression of the infection. This assay has allowed us to create a map of certain immune pathways, and show how cells in direct contact with the infection site respond, how cells further away respond, and which tissues don't respond. Further, we have started to analyze the plant's responses in a more natural environment, using 'real world' agricultural soil, rather than just laboratory medium or potting soil. This spatial immunity approach is new and highly promising to understand the plant immune system on an individual cell level.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Pathogen-caused diseases represent one of the biggest problems in agriculture. A detailed mechanistic understanding of plant defenses against pathogenic invasion and disease progression is therefore a vital research topic, forming the foundation for the creation of more resistant crop varieties. However, at present, most studies aimed at this utilize ‘omics’ approaches or mutant analyses, which lack resolution and only allow limited understanding of plant-pathogen interactions at the single-cell level. Thus, our current models of plant-pathogen interaction do not include strictly local or temporal responses. The aim of this proposal is to address this with a pathosystem consisting of the model plant Arabidopsis thaliana and strains of Fusarium oxysporum, a destructive fungal pathogen of several food crops. A unique near-native imaging setup, enabling the simultaneous study of growth and infection of plant and fungus with single-cell resolution, will be used. The work in this proposal will probe the responses of individual plant cells to both pathogenic and beneficial F. oxysporum strains, in a native tissue context from the onset of invasion to full disease development. This will generate novel insights into cellular mechanisms employed by plants to fight off pathogenic invaders, while accommodating beneficial endophytes. By using a novel in vivo cell-labeling technique, exploiting the fungal avirulence effector secretion system, a single-cell transcriptome atlas exclusively of cells undergoing acute colonization will be generated. Moreover, as root barriers represent a physical hindrance for pathogen invasion, mutants affected in physical defenses will be tested. Combined, this proposal will expand our current models of immune responses to include specific cells, regions or tissues, and temporal aspects with high resolution. Such knowledge is important to develop next-generation agricultural tools and will be applicable to combat current and arising diseases.

Оригинален текст от CORDIS (на английски).

Участници

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV · MUNCHENКоординаторГермания