HEИндивидуална стипендия2023–2025

LYSM · Unravelling the potential of LysM-domain proteins to awaken silent secondary metabolites genes in predatory bacteria through predator-prey interactions.

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-09-01 → 2025-08-31
Финансиране от ЕС: 165 313 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Протеините LysM при бактерията Myxococcus xanthus се изследват за това как помагат на хищника да разпознава и разгражда жертвите си. Това помага да се разбере как се активират специфични гени за производство на вещества, с които бактерията убива други микроорганизми.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Unravelling the potential of LysM-domain proteins to awaken silent secondary metabolites genes in predatory bacteria through predator-prey interactions.

LYSM project focuses on a group of uncharacterized proteins from the soil predator Myxococcus xanthus that are involved in predation of other microorganisms, and their potential involvement in the signalling cascade activating the normally silenced gene clusters for secondary metabolites production that are only expressed during this interaction. The research performed on the LysM proteins has proved that they are important for efficient predation on different prey organisms. These proteins (predicted to act as peptidoglycan hydrolases with different target sites on these macromolecules) have shown to act extracellularly improving predation of M. xanthus rather than directly acting on the prey killing process. Considering that deletion mutants of the lysM genes displays altered expression of some secondary metabolites genes, evidence so far suggests that LysM proteins may be linked to the prey-sensing process that allows M. xanthus to detect and discern between different microorganisms to select and produce the proper array of lytic factors to kill their prey. The data we have gathered so far has allowed us to build a model where the LysM proteins would degrade the peptidoglycan remains from the prey to generate peptidoglycan fragments that can be used as MAMPs (Microbial Associated Molecular Patterns) to be sensed by the predator.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

As a microbiologist with an expertise based on the study of the genetic response of microorganisms to their environment and other microorganisms, with a strong background in myxobacterial biology, I will study the induction of new antibiotic compounds in predator-prey co-cultures through the LYSM project. A transcriptome analysis of the predator bacterium Myxococcus xanthus in such conditions has revealed the induction of five genes coding for proteins with peptidoglycan-binding LysM domains together with several gene clusters involved in the production of secondary metabolites. The LYSM project will be carried out at the Department of Microbiology of the Universidad de Granada (UGR) under the supervision of Prof. José Muñoz Dorado. Their current line of research in bacterial predation and the experience I acquired throughout my international post-doctoral stages will create a strong synergy where we will achieve greater advances than we would separately. His research group counts with several experts in myxobacteria, bacterial predation, and transcriptional regulation, and an excellent network of international collaborators, that will contribute to the success of this project.This research project aims to discover mechanisms to “awaken” silent new secondary metabolites genes of M. xanthus through predator-prey interaction focusing on the role of peptidoglycan-binding proteins during predation.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSIDAD DE GRANADA · GranadaКоординаторИспания