ReversE · Modifying Enzyme with Solid-Binding Peptide for Site-specific and Reversible Enzyme Immobilization

Modifying Enzyme with Solid-Binding Peptide for Site-specific and Reversible Enzyme Immobilization

ReversE (Modifying enzyme with solid-binding peptide for site-specific and reversible enzyme immobilization) aims to address a key question, with a special focus on the longevity of the labile enzyme-based systems, which remains a bottleneck in enzyme-electrode biotechnological applications. Redox enzymes catalyze numerous biological processes, such as hydrogen evolution and uptake, carbon dioxide reduction, and nitrogen reduction, which are also relevant for a variety of applications, including enzymatic biofuel cells and the electrosynthesis of complex molecules. However, enzyme’s limited stability diminishes their potential to be exploited in biotechnological applications. The project’s overall goal is to develop a reversible immobilization approach that can attach enzymes on an electrode surface in a defined orientation and enable its controlled release on demand upon stimulation. This paves the way for enzyme regeneration on the electrode surface, which will be groundbreaking in the context of sustainable deployment of enzyme‒electrode systems. To accomplish this goal, the project is structured around three core objectives. Objective 1 – To genetically engineer redox metalloenzymes for site-specific immobilization Objective 2 – To establish methods for releasing bound enzymes from electrodes under mild conditions, preserving the properties of the electrode surface, and allowing new cycles of enzyme immobilization Objective 3 – To demonstrate the practical use of the reversible binding for device reusability and prolonged operation in energy-conversion applications Pathway to impact Scientific: the findings are crucial for fundamental understanding of intricate mechanisms behind the association and dissociation of solid-binding peptide-fused enzyme on the solid electrode surface. Technological: develop a blueprint for a reusable enzyme-electrode suitable for practical implementation in future technologies. Industrial & economic: enables the regeneration of devices, making future enzymatic biofuel cells and biosensor technologies more sustainable, which would otherwise be hampered by costly and elaborate surface modifications. Societal & environmental: the reuse of gold or other solid electrodes supports the objectives of the European Green Deal by minimizing environmental impact and decreasing reliance on critical raw materials.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз