HEИндивидуална стипендия2024–2025

LoAGOding · Stay home or go beyond: deciphering the mechanisms underlying ARGONAUTE1 loading and sRNA movement in plants

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2024-01-01 → 2025-12-31
Финансиране от ЕС: 165 313 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Процесите на зареждане на малките РНК в протеина ARGONAUTE1 се проучват, за да се разбере къде точно в клетката се активират те. Това помага да се разбере как растенията регулират развитието си, защитават се от вируси и издържат на стрес.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Stay home or go beyond: deciphering the mechanisms underlying ARGONAUTE1 loading and sRNA movement in plants

Small RNAs (sRNAs) are powerful regulators of gene expression and are essential for development, stress resilience and antiviral defence in plants. When loaded into an ARGONAUTE (AGO) protein, they act as a guide for AGO to silence a target gene. A central open challenge is that AGO1, the main sRNA effector in plants, operates across compartments: it is found in the cytoplasm, where it executes post-transcriptional gene silencing, and in the nucleus, where it loads micro RNAs (miRNAs, a type of sRNA), and it is associated with additional regulatory functions. Nonetheless, we still lack a clear mechanistic understanding of where other sRNAs are loaded into AGO1, how nuclear and cytoplasmic “loading environments” compete, and how this partitioning shapes sRNA mobility between cells. This project set out to establish a compartment-resolved view of AGO1 loading by: (i) dissecting sRNA loading when AGO1 is confined to the nucleus versus the cytoplasm, (ii) decipher the connection between miRNA biogenesis (D-bodies) and AGO1 loading complexes, and (iii) testing how the subcellular site of loading influences local and systemic sRNA activity.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Small RNAs (sRNA) are essential regulators of gene expression, controlling development and preserving genome integrity, all across eukaryotic organisms. To execute their final functions, sRNA are loaded into an ARGONAUTE (AGO) protein to guide through sequence complementarity the regulation of target genes. In plants, sRNAs are divided into small interfering RNA (siRNA) and microRNA (miRNA), and both can be loaded into AGO1 for cell-autonomous action, or move out of the cell to act non-cell autonomously. This proposal outlines a series of complementary experiments to mechanistically study the loading of sRNA into AGO1 and to understand how it contributes to the intra- and inter-cellular movement of sRNA in plants. By combining cell biology, RNA biochemistry, deep-sequencing analysis, nuclear fractionation methods, micrografting and super-resolution microscopy, this project aims at: i) characterize the nuclear and cytoplasmic sRNA loading complexes (LCs), ii) decipher the connection between sRNA biogenesis and LCs and, iii) determine how sRNA loading could affect the sRNA movement. The results obtained will advance the state of the art in plants in our understanding of AGO1 loading mechanism, the execution of the AGO1 function and sRNA movement. Studying these pathways goes beyond mere mechanistic knowledge, as sRNA movement is being exploited to modulate plant and pathogen gene expression towards improving crop productivity and enhancing stress/pathogen tolerance, a booming strategy known as Spray-induced gene silencing. Altogether, this project is expected to create a wealth of original information, providing a meaningful contribution to European excellence and competitiveness in the field.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE DE RECERCA EN AGRIGENOMICA CSIC-IRTA-UAB-UB · CERDANYOLA DEL VALLESКоординаторИспания