HEИндивидуална стипендия2023–2025

AURORA · Applying photon Up-conversion in ROtaxanes for super-Resolution microscopy Analyses

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-10-01 → 2025-09-30
Финансиране от ЕС: 195 915 €
Участници: 2
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Молекулярни машини, наречени ротаксани, се използват за превръщане на нискоенергийна светлина във високоенергийна чрез специален физичен процес. Това позволява създаването на микроскопия с висока разделителна способност, която вижда обекти под дифракционния лимит без нужда от специални химикали.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Applying photon Up-conversion in ROtaxanes for super-Resolution microscopy Analyses

AURORA investigates an unconventional strategy for the design of molecular machines by employing [2]rotaxane architectures as platforms for fully autonomous nanoscale assemblies. Within these systems, the ring components undergo spontaneous translational random motions, a mechanical behavior capable of modulating their optical response when coupled to the Triplet–Triplet Annihilation photon Up-Conversion (TTA-UC) mechanism. This photophysical process enables the conversion of low-energy incident photons into higher-energy emission and remains remarkably efficient under low-intensity irradiation from standard, commercially available LED sources. A key point of the project is the intentional integration of nanoscale mechanical dynamics with tailored photophysical functionality. This coupling was anticipated to generate intermittent fluorescence bursts, an optical “blinking” behavior, that can be exploited to achieve imaging resolutions surpassing the classical diffraction limit. Building on this effect, the resulting interlocked assemblies were designed as functional probes for a variant of single-molecule localization microscopy, designated “T2-STORM,” which enables super-resolution imaging without reliance on specialized chemical buffers or strictly regulated experimental conditions. The AURORA research program is structured around three objectives: 1. Embedding the TTA process directly within rotaxane frameworks to achieve efficient photon upconversion. 2. Modulating stochastic emission events to improve “blinking” effect. 3. Deploying TTA-UC-active rotaxanes as probes for the T2-STORM high-resolution microscopy method.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

This multidisciplinary AURORA project focuses on the development of prototype rotaxane (molecular ring-on-thread) structures displaying triplet fusion upconversion photoprocess. Structural design will instill this process via spontaneous excited dye collisions in confined trajectories of the designed interlocked molecules to create a fluorescent state (from two combined low energy photons). Such architectures will help to collect information on molecular photon upconversion and intramolecular diffusion processes through time-resolved spectroscopies. Moreover, it will produce intermittent fluorescence blinking by which detailed analysis will afford a new methodology for super-resolution imaging, namely below the diffraction limit of light. In theory this can provide solutions to some limitations of actual approaches and impact this research field. Fluorescence modulation within these autonomous [2]rotaxanes by triplet fusion will lead to studies down to the single-molecule level (based on Stochastic Optical Reconstruction Microscopy) which will be employed to establish a new localization microscopy method (T2-STORM) and be validated through imaging commercial nanostructured biomacromolecules. Both leading French research institutes, Institut des Sciences Moleculaires (ISM) and Laboratoire Photonique Numerique et Nanosciences (LP2N) will co-host the AURORA project. The French fellow, specialist in synthetic organic and supramolecular chemistry, will be taught molecular photophysics/photochemistry and super-resolution fluorescence imaging. Additional work packages will guarantee efficient scientific advances, training, career development, management, communication and potentially technology transfer. Association of the fellow and research groups skills at the chemistry-photophysics-imaging interface, available infrastructure and equipment, will participate to both the fellows future independent research career and achieving ambitious AURORA objectives.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITE DE BORDEAUX · BordeauxКоординаторФранция
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS · ParisФранция