HEИндивидуална стипендия2023–2025

TraFICa · Transcription Factor-mediated Induction of Cardiomoycyte proliferation.

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-05-16 → 2025-05-15
Финансиране от ЕС: 187 624 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Сърдечните клетки при възрастните почти не се умножават, затова се търси комбинация от протеини, която да стимулира техния растеж в 3D модели. Това е важно, защото може да помогне за възстановяването на сърцето след инфаркт, когато част от тъканта е унищожена.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Transcription Factor-mediated Induction of Cardiomoycyte proliferation.

The adult heart has an extremely limited regenerative capacity because heart cells do not multiply (proliferate). A major ischaemic event such as a Myocardial Infarction (MI) which results in the loss of heart cells and the formation of fibrotic scar tissue, diminishing the contractile function of the heart. This pathological process can culminate in heart failure, with the only effective treatment being heart transplantation. Therefore, regeneration of the heart is a potential therapy for treatment of ischaemic heart disease. Firstly, TraFICa aims to develop a high throughput screening platform to study heart cell proliferation in 3D structures, grown from stem cells, that recapitulate the human heart. Next, this screening platform will be used to Identify a protein cocktail that stimulates proliferation in heart cells within these 3D structures. Finally, we will validate the protein cocktail in an ex vivo myocardial tissue culture system.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Heart failure is a major global public health problem affecting over 23 million patients worldwide. There are no effective therapies for heart failure that regenerate the damaged heart muscle following injury such as a myocardial infarction. The adult mammalian heart has an extremely limited intrinsic capacity for regeneration due to the fact that mature cardiomyocytes are unable to proliferate. To date, attempts to re-induce proliferation in mature cardiomyocytes of the adult heart have not yet been translated into the human clinical context. Much remains to be learned about molecular mechanisms controlling cardiomyocyte proliferation in humans if the therapeutic potential for cardiac regeneration is to be realised. With my extensive training and expertise in molecular and stem cell biology I plan to generate and exploit an innovative live cell cycle reporter-based screening platform to identify Transcription Factors (TFs) that can be induce cardiomyocyte proliferation. The three major objectives of this research proposal are: 1. Develop a high-content screening system to monitor cardiomyocyte proliferation in 3D human cardiac organoids, which will then be used to:2. Identify (combinations of) TFs that drive the human cardiomyocyte cell cycle.3. Assess the therapeutic potential of TF activation for cardiac regeneration in a pre-clinical human cardiac model system. I will receive extensive training in the ex vivo cardiac tissue culture system (objective 3), which will complement my proven expertise in the generation of (genetically modified) 3D cardiac organoids and knowledge on cell cycle regulation (objective 1 & 2). Altogether, the tool development, results and training obtained during this project will put me in the forefront of the cardiac regenerative field.

Оригинален текст от CORDIS (на английски).

Участници

ACADEMISCH ZIEKENHUIS LEIDEN · LeidenКоординаторНидерландия