HEИндивидуална стипендия2023–2025

ACO-LC · Applying ACOustic streaming to enhance mass transfer in Liquid Chromatography of large molecules

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-09-01 → 2025-08-31
Финансиране от ЕС: 175 920 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Звуковите вълни се използват за подобряване на разделянето на големи молекули, като например вариантите на хемоглобина. Това позволява по-точно измерване на гликирания хемоглобин (HbA1c), което помага за по-доброто проследяване и диагностициране на диабета.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Applying ACOustic streaming to enhance mass transfer in Liquid Chromatography of large molecules

High performance liquid chromatography (HPLC) is the most widespread analytical separation technique. The unique characteristics, make HPLC an indispensable tool in biological and pharmaceutical research, clinical diagnostics, and food quality inspection. Point-of-care (POC) testing is also a growing domain leading to the transition of diagnostics from the hospital to the primary setting. Diabetes is a major cause of blindness, kidney failure, heart attacks, stroke, lower limb amputation, and leads to premature deaths. At present, three methods are recognized for monitoring blood sugar, and consequently, diabetes diagnosing. The level of glycated hemoglobin (HbA1c) has been shown to assess long-term glycemic control and is the best marker for the risk of developing chronic complications of diabetes. However, neither the currently available routine laboratory methods nor POC tests enable to provide high quality HbA1c analysis and accurate quantification of other hemoglobin variants within a single run. The present project introduces a novel methodology to improve the separation efficiency of large molecules such as Hb variants, which is based on vortex generation inside the separation column using acoustic waves. This vortex-LC method enhances separation efficiency through the reduction of sample dispersion, and consequently, the separation resolution improves allowing for the quantification of HbA1c more accurately.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Liquid chromatography (LC) is a prominent analytical technique serving life-sciences, chemical and pharmaceutical sciences, which plays a key role in virtually all research and development concerned with liquid phase (bio)chemistry. The performance of pressure-driven LC has continuously increased during the past century, with improvements realized by ordering the packing and reducing the particle size. Now that the point is reached where sub-micron ordered packings can be reliably produced, the performance limitation of LC lies in the principle itself, more precisely in the slow mass transport of analytes between the field lines with varying cross-sectional Poiseuille velocity vectors. Distribution of analytes in the sample bands over different local velocities results in Taylor-Aris dispersion, an effect that becomes increasingly important at high velocities, or at low diffusion coefficients. ACO-LC addresses this lateral diffusion limitation by inducing acoustically generated lateral flows obtained in resonance mode. The resulting vortices in this so-called acoustic streaming regime carry the analytes much faster than purely diffusive processes, therewith reducing the dispersion dependency on size and mobile phase velocity. While the efficacy of this acoustic vortex LC methodology has been recently shown in microfluidic channels in the host group, the channel dimensions need to be scaled to the micron-scale range, with the resonance condition maintained. In ACO-LC, innovative geometrical approaches will be pursued to overcome frequency limitations of commercial piezoceramic actuators. Improved separations of glycosylated hemoglobin variants are aimed for in glass substrates, which are compatible with integrated UV-VIS absorption. This improved separation application is of interest to millions of diabetes patients for routine separations and tackles an additional separation challenge encountered in multi-ethnic groups.

Оригинален текст от CORDIS (на английски).

Участници

VRIJE UNIVERSITEIT BRUSSEL · Bruxelles / BrusselКоординаторБелгия