HEИндивидуална стипендия2024–2026

LyticPol · Understanding the new oxidative paradigm of biomass waste upcycling

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2024-04-01 → 2026-03-31
Финансиране от ЕС: 214 934 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

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Накратко на български

Ензимите LPMO разграждат растителни отпадъци чрез окисление, но механизмът на действие на медта в тях остава неясен. Разбирането на този процес помага за оптимизиране на индустриалното производство на биогорива и химикали, които да заменят изкопаемите горива.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Understanding the new oxidative paradigm of biomass waste upcycling

Tackling the climate crisis requires replacing fossil fuels with sustainable alternatives. An example of a renewable feedstock is lignocellulosic biomass. Its use has several advantages: since it is obtained, for example, from forest and agricultural residues, it does not compromise global food security, and it can be used to produce biofuels, as well as bioproducts, and more than 200 different chemicals. However, lignocellulose is a very recalcitrant material, hampering its use as a direct replacement for fossil fuels. A promising way forward is the use of enzyme cocktails to break down lignocellulosic biomass. Important components of such cocktails are the enzymes lytic polysaccharide monooxygenases (LPMOs). To date, eight different LPMO families have been discovered. All eight families contain a single copper atom, which allows them to employ a unique oxidative mechanism to break down several polysaccharides. Yet, this mechanism remains elusive because too few LPMO families have been considered in previous studies, and the theoretical methods employed have been insufficient. The proposed “LyticPol” project aimed to develop a novel theoretical method and employ it on a large set of representative LPMO structures. The main scientific objective was to obtain a more general picture of the LPMO mechanism, as a basis for fine-tuning the industrial reaction conditions for LPMOs. Overall, the project aimed to contribute decisively to the career goal of the researcher: to become an independent and innovative researcher in the field of bioinorganic chemistry.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

As the EU is committed to becoming the world’s first climate-neutral continent, fossil fuels need to be replaced by sustainable alternatives. A potential alternative is the upcycling of lignocellulosic biomass to biofuels. However, the cost-efficient generation of biofuel from cellulose is considerably hindered by the recalcitrance of the cellulose biopolymer. In 2010, the discovery of a new family of copper-dependent metalloenzymes called lytic polysaccharide monooxygenases (LPMOs) spurred great hopes as LPMOs were shown to significantly boost the degradation of cellulose and other polysaccharides.Today, eight families of LPMOs have been classified with remarkably diverse substrate specificities and regioselectivities. Furthermore, the metal active sites are in rather different surroundings. Despite combined efforts from experimental and theoretical sides, the reaction mechanism of LPMOs has remained elusive. The here proposed “LyticPol” project suggests that previous theoretical studies lacked general conclusions because they (i) considered too few LPMO families and (ii) employed insufficient theoretical methods for the enzyme environment.The “LyticPol” project aims at elucidating the reaction mechanism of LPMOs, by using a novel theoretical method on a large set of representative LPMO structures. From knowing the mechanism, the reaction conditions of LPMOs can be fine-tuned and the full industrial potential can be reached. This is expected to have substantial economic and social impact. Hence, the results of the “LyticPol” project will be communicated not only to scientific experts but also to the general public. The project will be carried out by Dr. Erna K. Wieduwilt (EKW) under the supervision of Prof. Erik D. Hedegård at the University of Southern Denmark. The project itself and the training of EKW will contribute decisively to her career goal of becoming an innovative and independent researcher in the field of theoretical bio-inorganic chemistry.

Оригинален текст от CORDIS (на английски).

Участници

SYDDANSK UNIVERSITET · Odense MКоординаторДания