HEИндивидуална стипендия2023–2025

SCOBY DO · Symbiotic Culture of Bacteria and Yeast Ecosystems for the development of Probiotic Extracellular Vesicles

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-10-01 → 2025-09-30
Финансиране от ЕС: 188 590 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Микробите в комбучата комуникират чрез екстрацелуларни везикули – малки структури, които пренасят протеини и генетичен материал. Разбирането на този процес помага при разработването на средства за борба с бактериалните инфекции в стомашно-чревния тракт.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Symbiotic Culture of Bacteria and Yeast Ecosystems for the development of Probiotic Extracellular Vesicles

The SCOBY-DO (Symbiotic Community of Bacteria and Yeast – Discovery and Outreach) project, funded under the Horizon 2022 -MSCA (Marie Skłodowska-Curie Actions), explores microbial communication using kombucha as a model system. Kombucha is a traditional fermented tea produced by a symbiotic culture of bacteria and yeast. This microbial ecosystem is both resilient and dynamic, offering a natural laboratory for studying microbial cooperation, competition, and stability over time. The project addresses a key scientific challenge: understanding how microbes communicate through extracellular vesicles (EVs). EVs are nanoscale, membrane-bound structures that transport proteins, nucleic acids, and metabolites. Although their role is increasingly recognised in human biology and medicine, little is known about their ecological role in natural microbial consortia. The main objectives of SCOBY-DO are: 1. Optimize SCOBY kombucha model system, kombucha-derived single cultures and binary co-cultures to produce EVs. 2. Study i.) Kombucha-derived EVs as potential therapeutic agents in gastrointestinal bacterial infection and ii.) the role of SCOBY EVs in SCOBY cross-species communication iii.) to design and synthetize polymers through the molecular imprinting technologies (MITs) for the specific recognition or release of kombucha-derived EVs. These objectives align closely with MSCA’s priorities by combining excellent science, interdisciplinary training, and societal engagement.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Fermented foods are used for many centuries all over the world for their contribution to longevity and digestive health. Regular consumption of fermented foods can combat dysbiosis and boost hosts immune response against various pathogens and associated diseases. However, they increase the microbiome of the digestive tract and can have adverse effects on immunosuppressed patients. Rather than single-strain probiotics, we will study Symbiotic Culture of Bacteria and Yeast Ecosystem (SCOBY) derived biomembrane-delimited nanostructures to obtain similar or enhanced beneficial effects. To that end, extracellular vesicles (EVs) harvested from kombucha, a fermented beverage based on tea and sugar produced with a SCOBY will be exploited for their antimicrobial properties against gastrointestinal bacterial infections and food-borne bacterial pathogens.The experienced researcher is a microbiologist with strong academic background in the field of microbiology, infectious diseases, and bioinformatics who will team-up with the Institute of Biosciences and BioResources with considerable expertise in EV research to carry out a uniquely interdisciplinary research program. The research will realize the following objectives: 1. to set-up and optimize kombucha SCOBY cultures for the production of EVs, 2. to study the role of SCOBY-derived EVs in interspecies communication and their impact on gastrointestinal bacterial pathogens, and 3. to synthetize molecularly imprinted polymers specific for the enrichment of SCOBY-EVs. To our knowledge, none of these objectives has been addressed before and they all have the potential to expand the knowledge in the field and drive the research towards biotechnology applications in health and disease. The research objectives are integrated with concerted training objectives in EV research, mass spectrometry, MIPs design and synthesis, outreach program, dissemination events and considerable knowledge transfer in microbiology from the researcher.

Оригинален текст от CORDIS (на английски).

Участници

CONSIGLIO NAZIONALE DELLE RICERCHE · RomaКоординаторИталия