HEИндивидуална стипендия2023–2025

COOLCASE · Uncovering the regulatory landscape of Arabidopsis thaliana responses to cold temperatures

„Хоризонт Европа“ — Действия „Мария Склодовска-Кюри“

Период: 2023-12-01 → 2025-11-30
Финансиране от ЕС: 165 313 €
Участници: 1
Схема: HORIZON-TMA-MSCA-PF-EF

Линиите свързват координатора с партньорите.

Накратко на български

Генната активност на растението Arabidopsis thaliana се проследява при спад на температурите, за да се разбере как се променя достъпът до ДНК. Това помага да се разберат механизмите за оцеляване при студ и да се създадат по-устойчиви култури.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Uncovering the regulatory landscape of Arabidopsis thaliana responses to cold temperatures

Due to global climate change, the low temperatures generally observed during winter are dramatically decreasing whereas the first warm days of spring are now often followed by sudden extreme cold spells. Understanding how plants perceive and respond to prolonged cold exposure under such fluctuating conditions is therefore crucial. The global objective of the COOLCASE project was to investigate, for the first time, the behavior of Arabidopsis thaliana transcripts over weeks of decreasing temperatures, mimicking what plants can encounter in their natural environment. The project aimed to correlate gene expression with chromatin accessibility, highlighting the critical roles of transcription factors and gene regulatory networks in plant cold acclimation, while also functionally validating selected candidate genes. By combining transcriptomic and epigenomic approaches, COOLCASE provides novel insights into plant adaptation to natural cold regimes. A deep understanding of the regulatory aspects of the Arabidopsis transcriptome during cold acclimation sheds light on the molecular programs plants have evolved to survive harsh winter conditions and reveals new loci for potential breeding of more resilient crops.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Plants, as sessile organisms, must constantly adapt to variable environmental conditions, among which temperature is of paramount importance in the context of climate change. Indeed, in future years, despite an expected rise in temperatures due to global warming, we can also expect an increase in the frequency of severe cold periods for which plants are not prepared. Therefore, understanding the molecular processes that allow plants to react and adapt to cold is essential for developing cold-resistant plants, which is ultimately indispensable for global food security. In recent decades many studies have embarked in addressing this issue. However, the global regulatory landscape underlying cold acclimation and adaption of plants to noisy and fluctuating natural cold conditions remain poorly investigated. Low temperatures are known to have a direct effect on chromatin accessibility which is directly linked to gene expression. Therefore, the aim of this project is to investigate the dynamics of the coding and non-coding Arabidopsis thaliana transcriptome, as well as changes in chromatin accessible sites, in response to regimes of decreasing temperatures. To this end, a comparative analysis of deep-coverage RNA-seq (transcriptome) and ATAC-seq (chromatin accessibility) will be performed on A. thaliana plants grown over weeks of fluctuating temperatures, mimicking natural cold conditions of autumn and early winter. The proposed experimental design will reveal novel coding genes, long non-coding RNAs (lncRNAs) and potential distal regulatory elements involved in Arabidopsis responses to cold. Thus, by combining interdisciplinary and mutually reinforcing approaches including (epi)genomics, (epi)genetics, transcriptomics and bioinformatics, the proposed project will significantly improve our understanding of the molecular mechanisms required for plant adaptation to cold temperatures likely delivering valuable tools for future breeding of cold resilient crop species.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE DE RECERCA EN AGRIGENOMICA CSIC-IRTA-UAB-UB · CERDANYOLA DEL VALLESКоординаторИспания