Immunocytological and genetic characterization of cytoplasmic dynein heavy chain gene products in mouse
4РП — Обучение и мобилност на изследователи
- Период
- 1997-05-01 → 1998-04-30
- Финансиране от ЕС
- —
- Участници
- 2
- Схема
- RGI
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Цитоплазматичният динеин е протеин, който транспортира органили в клетката и помага при деленето на ембрионите на мишките. Анализът на мутации в този протеин помага да се разберат механизмите на клетъчното делене и функцията на специфични гени.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Цел на проекта
Cytoplasmic Dynein is a microtubule-associated and -activated ATPase which is thought to bind to the surface of membranous organelles and kinetochores of mitotic chromosomes toward the microtubule 'minus' ends and is responsible for retrograde axonal transport. In Dr. Chalepakis laboratory, a mouse mutant which lacks the gene for the heavy chain of cytoplasmic dynein (Dychc), is available. Homozygous mutant mice die at the late morula stage, approximately after five cell divisions of the fertilised oocyte. These dividing embryo cells provide an ideal working system to study the mitotic spindle in the absence of functional cytoplasmic dynein. Moreover, it will be very interesting to decipher the cellular mechanisms that lead to the five divisions of homozygous mutant embryo cells which lack dynein. In parallel to this, a new putative cytoplasmic dynein heavy chain gene (DLP4) will be characterised and its subcellular distribution will be compared to that of Dychc by using immunocytological methods. The goals of my project lie within the interests of the host lab. The choice of the host lab seems appropriate because my previous training at EMBL onmodern cell biology techniques, immunology, electron microscopy and genetic analysis, will be meaningfully complemented by the expertise and know-how of Dr. Chalepakis lab. in developmental-molecular biology approaches. This can provide a basis for a productive give and take" with the right blend of freedom and guidance. I also believe that I can contribute with my knowledges in the undergraduate as well graduate Programmes of the department of biology. "
Оригинален текст от CORDIS (на английски).
Участници
Връзки
Данни: CORDIS, © Европейски съюз