Enzymatic synthesis and activation of sialic acid analogs
4РП — Обучение и мобилност на изследователи
- Период
- 1997-08-01 → 1999-07-31
- Финансиране от ЕС
- —
- Участници
- 2
- Схема
- RGI
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Ензимни процеси създават аналози на сиаловата киселина, като например сериови производни на захари чрез генетично модифицирани бактерии. Това помага за изучаване на биологичните свойства на тези молекули и техните функции в организма.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Цел на проекта
Research objectives and content The main objective of this research is to investigate the NeuAc system. This would require the parallel subcloning of two plasmid-based E. coli genes for synthesis (N-acetyl neuraminic acid synthase = NeuS; EC 4.1.3.19) and activation (CMP-N-acetyl neuraminic acid synthetase = CMP-NeuS; EC 2.7.7.43) of neuraminic acid analogs into a commercial expression vector under the control of a strong inducible promoter (e.g., pKK223-3; Pharmacia), as well as the overexpression, purification, and characterization of the corresponding enzymes for preparative applications. Since only few ManNAc analogs are commercially available for the synthesis of sialic acid analogs the first step is to synthesize selected ManNAc derivatives or analogs. In this sense, it would be interesting the synthesis of the thio derivative: that can be very useful in the study of the NeuAc system and in the synthesis of 6-thio-N-acetyl-Dneuraminic acid derivatives. Training content (objective, benefit and expected impact) The synthesis of sialic acids analogues presents an important problem in view of the interesting biological properties that can be anticipated. As well as the synthetic and analytical techniques, with which I have become familiarized during my PhD, this work would include basic techniques of gene technology, protein chemistry, and enzyme-catalyzed synthesis of nucleotide-activated sugars and oligosaccharides, allowing me to use a wide range of methods.
Оригинален текст от CORDIS (на английски).
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Данни: CORDIS, © Европейски съюз