Origin usage in a human cell-free system
4РП — Обучение и мобилност на изследователи
- Период
- 1998-01-12 → 2000-01-11
- Финансиране от ЕС
- —
- Участници
- 2
- Схема
- RGI
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Механизмите за стартиране на репликацията на ДНК при хората се анализират чрез специална система извън живата клетка. Това помага да се разберат молекулярните процеси при предаването на генетичната информация и начина, по който се контролира S-фазата на клетъчния цикъл.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Цел на проекта
Research objectives and content DNA replication is a process of dramatic interest for its relevance in the transmission of genetic information from one cell generation to the next. Unfortunately, this process in human cells is still poorly known. However, very recently, the first human system that supports replication in vitro has been developed in R.A.Laskey laboratory. In this cell-free system nuclei from G1 phase HeLa cells initiate semiconservative DNA replication in the presence of cytosolic and nuclear factors from S-phase cells. Exploiting the skills and the knowledge of the laboratory that developed the cell-free system, I propose to utilize the same to understand the nature of DNA replication initiation in human cells. Basically, I will utilize three approaches: - origin usage studies including replication timing analysis and mapping of origins at the DNA level - replication assays using different templates to establish an in vitro system able to replicate not only DNA presented as Gl nuclei, but also DNA provided as another source - biochemical approaches including fractionation, addition and selective immunoprecipitation of target proteins. These approaches will allow me to evaluate the importance of DNA sequences versus chromosomal and nuclear structures in selection of replication origins and to explain the roles of initiation proteins. Training content (objective, benefit and expected impact) The newly developed human cell-free system will be crucially important in the dissection of the S-phase cellular controls as well as the molecular mechanisms underlying DNA replication. Moreover, the same will be an important approach to study other relevant cellular processes such as DNA recombination and repair. Links with industry / industrial relevance (22) Possible links with the industry could be the use of this assay in investigating the effects of anti-tumor drugs and also the effects of different stimuli, such as X-ray irradiations, on the control mechanisms of DNA replication and, as a consequence, on
Оригинален текст от CORDIS (на английски).
Участници
- University of Cambridge · CambridgeКоординаторОбединеното кралство
- Not availableНиво градИталия
Връзки
Данни: CORDIS, © Европейски съюз