Study of the role of the coagulation system in blood vessel development and disorders via generation of transgenic mice
4РП — Обучение и мобилност на изследователи
- Период
- 1998-02-01 → 2000-01-31
- Финансиране от ЕС
- —
- Участници
- 2
- Схема
- RGI
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Системата за съсирване на кръвта и протеинът TF се изследват чрез генетично модифицирани мишки, за да се види как влияят върху развитието на кръвоносните съдове. Това помага да се разбере как се формират съдовете при ембрионите и как се развиват съдовите нарушения при възрастните.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Цел на проекта
Research objectives and content The coagulation system plays an essential role in hemostasis via generation of fibrin. However, recent evidence suggests that this proteinase system may participate in other biological processes. Indeed, we recently demonstrated that targeted inactivation of the cellular tissue factor (TF) receptor results in embryonic lethality due to abnormal vascular development. Whether TF exerts a morphogenetic role during blood-vessel formation remains to be determined. Factor VII (FVII) is the only known ligand for TF but data resulting from the generation of FVII -/- mice suggest that TF may act independently of the hemostatic properties of FVII during vascular development. The specific aims of our project are: 1) to study the in situ expression of TF and FVII in blood-vessel development 2) to generate transgenic mice expressing a wild type TF or a carboxy-terminally truncated TF transgene in a tissue-specific pattern in order to 'rescue' the embryonic lethality associated with TF deficiency. This will allow to study the role of TF beyond the initial stage of embryogenesis 3) to generate transgenic mice in which TF transgene will be expressed but can be still inactivated in a temporo-spatial pattern allowing to study the consequences of TF deficiency during adulthood angiogenesis. Training content (objective, benefit and expected impact) This project will allow me to learn not only new molecular biology techniques (e.g. generation of transgenic mice and inducible system (CRE/LoxP)) but also in vivo angiogenesis models (wound healing, neovascularisation of the ischemic heart or during ischemic retinopathy). All these techniques, already available in the host institution, will complete my previous knowledge on blood-vessel formation gained in the course of my Ph.D. Links with industry / industrial relevance (22) see description of host institution.
Оригинален текст от CORDIS (на английски).
Участници
Връзки
Данни: CORDIS, © Европейски съюз