Functional and molecular differences between hippocampal synapses studied at the single synapse level
4РП — Обучение и мобилност на изследователи
- Период
- 1998-11-01 → 2000-10-31
- Финансиране от ЕС
- —
- Участници
- 1
- Схема
- RGI
Линиите свързват координатора с партньорите.
Накратко на български
Синапсите в хипокампуса на плъхове се анализират поотделно, например чрез проследяване на протеини и електрически токове. Това помага да се разбере функционалното разнообразие между отделните връзки в мозъка и как те се променят при пластичност.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Цел на проекта
Research objectives and content Synaptic transmission in the mammalian central nervous system has been analysed traditionally via electrophysiological recordings obtained from the neuronal cell body. Using this methodology, the funtional diversity of individual synapses cannot be sufficiently described. Here, different methods suited to monitor events at the single synapse level will be applied to rat hippocampal neurons.Loose patch recordings of spontaneous miniature postsynaptic currents, so far succesfully employed for the analysis of individual excitatory synapses, will be extended to inhibitory synapses. Therby, precise informations concerning principal parameters of these synapses can be obtained; as, for example, the extent of receptor saturation by a quantum of neurotransmitter.While loose patch recordings mainly focus on the postsynaptic site, also presynaptic events will be monitored at single synapse resolution. The distribution of the synaptic vesicle protein synaptotagmin and potentially of other presynaptic proteins before and after the induction of different forms of synaptic plasticity will be followed in real time with various fluorescent labelling methods. These experiments may reveal plasticity-related changes in the protein composition of individual synapses. Training content (objective, benefit and expected impact) Training will be devoted to new and powerful electrophysiological and imaging techniques developed by the host laboratory. Adding to already present experience in the characterization of synaptic proteins, learning those methods and discussing state-of-the-art concepts of synaptic transmission will significantly strengthen personal expertise. Links with industry / industrial relevance (22) The host laboratory of Dr. Malgaroli has a long-standing collaboration with Glaxo Geneva.
Оригинален текст от CORDIS (на английски).
Участници
- FONDAZIONE CENTRO SAN RAFFAELE DEL MONTE TABOR - ISTITUTO DI RICOVERO E CURA A CARATTERE SCIENTIFICO · MILANOКоординаторИталия
Връзки
Данни: CORDIS, © Европейски съюз