ENDOREPTOM · Functional analysis of endoreduplication during tomato fruit development.
6РП — Действия „Мария Кюри“
- Период
- 2004-07-01 → 2005-06-30
- Финансиране от ЕС
- 40 000 €
- Участници
- 1
- Схема
- ERG
Линиите свързват координатора с партньорите.
Накратко на български
Ролята на протеина WEE1 при развитието на плода на домата се анализира чрез проследяване на деленето и разширяването на клетките. Разбирането на тези процеси помага за определяне на факторите, които влияят върху размера и качеството на плодовете.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - ENDOREPTOM (Functional analysis of endoreduplication during tomato fruit development)
The control of the two developmental processes that are cell division and cell expansion is a crucial determinant of fruit size, an essential criterion for final fruit quality. Indeed, in many species the variation in final fruit size has been attributed to differences in cell number rather than cell size. The cell number is thus a key component in the determination of tomato fruit size. According to the fruit developmental scheme, genes coding for cell division regulators such as cell cycle proteins may represent relevant candidate genes which could be involved in the molecular mechanisms governing fruit organogenesis. Among these regulators, the WEE1 protein kinase is involved in the control of cell size checkpoint of the cell cycle in fission yeast, preventing the cell to engage into mitosis unless it reaches a minimal cell mass. This project aimed at investigating the role for WEE1 in higher plant cell size determination. In plants WEE1 is encoded by a single gene which makes it an interesting candidate to manipulate in planta, and especially in fruits. We thus investigated the regulation of the WEE1 gene in tomato. First we cloned the complete genomic and cDNA sequences for WEE1. We then studied the expression profile of the WEE1 gene as determined by semi-quantitative RT-PCR in whole fruits and in the dissected tissues of the fruit at the various stages of fruit development and in the different vegetative organs of tomato plant. These data indicated that the WEE1 gene expression was enhanced during the cell expansion phase of tomato fruit development, especially within the gel tissue when mitosis is hampered and cell polyploidisation through endoreduplication is triggered. To perform a functional analysis, transgenic tomato plants were generated in which the expression of WEE1 is inhibited by producing antisense RNA. Inhibiting the WEE1 expression resulted in an alteration of plant size, fruit size and cell size, together with a decrease in cell polyploidisation. Our data bring further knowledge about the control of cell cycle, cell size and endoreduplication in higher plant cells.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Endoreduplication (somatic polyploidy) is a developmental process described in many eukaryotes. This phenomenon represents a growing field of interest in plant biology since it characterises the switch between cell proliferation and cell differentiation during development.However its physiological role remains largely unknown. In order to study the molecular mechanisms governing the transition between mitosis and endoreduplication, we will take advantage of tomato fruit as a biological model in which pericarp tissue can reach a ploidy of 512C. This project is based on a candidate gene approach, namely the WEE1 gene.The phosphorylation target of the WEE1 kinase is the Cyclin-Dependent-Kinase (CDK) subunit of mitotic CDK/Cyclin complexes, thus inactivating their activity. To characterize the function of WEE1, we will test its ability to inhibit in vivo CDK/Cyclin kinase activity. The putative interactors of WEE1 will be searched by a two-hybrid screen. The in planta functional analysis of WEE1 will be assessed by characterizing wee1-antisense tomato plants previously obtained in the host lab. The originality of the project resides in an integrative approach to combine transcriptome and metabolome analysis of wild type and wee1-antisense plants for deciphering the role of WEE1 and endoreduplication in fruit development and composition. Importantly, the project benefits both from the expertise that the applicant acquired during his mobility period, and from the host laboratory leading position in the plant endoreduplication field. New insights resulting from this reintegration programme will form a strong basis for a long-term project in studying the physiological role of endoreduplication, through a permanent position inside the host laboratory. The expected results should contribute to our general understanding of the endoreduplication contribution in the elaboration of the final fruit size, and to improve the current knowledge in fruit development and quality.
Оригинален текст от CORDIS (на английски).
Участници
- INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE · PARISКоординаторФранция
Връзки
Данни: CORDIS, © Европейски съюз