GIEGE-ERG · Cytochrome c biogenesis in higher plant mitochondria
6РП — Действия „Мария Кюри“
- Период
- 2004-01-20 → 2005-01-19
- Финансиране от ЕС
- 40 000 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите.
Накратко на български
Митохондриите в растенията използват цитохром c за транспорт на електрони, като неговото създаване изисква свързване на хем към протеин. Разбирането на този механизъм помага да се разбере как функционират енергийните станции в растителната клетка.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - GIEGE-ERG (Cytochrome c biogenesis in higher plant mitochondria)
Mitochondria are considered to be power stations in the plant cell. In mitochondria, the respiratory chain uses cytochrome c as an electron transporter. These cytochromes distinguish themselves by the covalent attachment of the heme to the apoprotein. Altogether, the terms 'maturation' or 'biogenesis' of cytochrome c describe all the mechanisms comprising the binding of the heme to the apocytochrome, the transport of both components to their assembly site, the inter membrane space as well as protein and heme reduction mechanisms necessary for the covalent binding of the heme. Surprisingly, in plant mitochondria, cytochrome c are assembled by a complex mechanism, similar to the one described in some bacteria. Several proteins involved in this pathway have already been identified by their sequence similarity with bacterial cytochrome c maturation proteins (CCM proteins). However, many proteins predicted by the bacterial model are yet to be characterised in plant mitochondria. During this project the following results have been obtained: The analysis of protein complexes containing CCM proteins by BN-PAGE has shown the existence of 480, 900, 500 and 500 kDa that can be in interaction with complexes of the respiratory chain Protein-protein interactions of CCM proteins have been studied. These experiments show that there are interactions of CCMH with apocytochrome c and also some interactions between CCMA and CcmB, the components of an ABC transporter. An appreciation of the expression of ccm genes has been done using a CATMA microarrays and is now analysed. Further analysis will now be done in the laboratory.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Abstract: The research program aims at studying a mechanism specific of land plant mitochondria: the biogenesis of c type cytochromes. This mechanism taking place in the mitochondria inter membrane space is performed by a multi-submits protein complex composed of subunits encoded in both the nuclear and the mitochondria genome. The first goal of the research will be to purify this complex and to characterise its subunits. This project also fits in the wider problematic of gene expression coordination between the nucleus and mitochondria. This study was initiated during the post-doctoral training course financed by a Marie Curie fellowship. The particular case of the cytochrome assembly complex and its relation with the rest of the plant cell will preferentially be used as a model during this study. The study of cytochrome c biogenesis is also very interesting in the context of the recent discovery of the involvement of cytochrome c in the triggering of programmed cell death (PCD). It is possible that this metabolism could act as a regulator in some PCD mechanisms involving cytochrome c. DNAmicroarray technology will be used to investigate the expression regulations of cytochrome c biogenesis genes and the relations of this mechanism with other mechanisms in the plant cell, particularly PCD. This research project, with its purpose of answering precise biological questions, will use biochemicals well as genetic techniques. It will also allow the implementation and adaptation of advanced technologies in the laboratory of origin, in particular polemics and DNA micro arrays, to the research field of plant mitochondria.
Оригинален текст от CORDIS (на английски).
Участници
- CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · PARISКоординаторФранция
Връзки
Данни: CORDIS, © Европейски съюз