T-CELLS AND SHIGELLA · T-cell immune response and T-cell apoptosis in Shigella infection
6РП — Действия „Мария Кюри“
- Период
- 2005-04-01 → 2007-03-31
- Финансиране от ЕС
- 158 218 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите.
Накратко на български
Имунният отговор на Т-клетките при инфекция с бактерията Shigella се проучва чрез мишарски модел, като се следи действието на специфичните Th17 клетки. Резултатите помагат за разбирането на защитните механизми на организма и подобряването на стратегиите за създаване на нови ваксини.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - T-CELLS AND SHIGELLA (T-cell immune response and T-cell apoptosis in Shigella infection)
Shigellosis is an acute infection of the human colon causing dysentery characterised by pain, fever, and severe bloody diarrhoea. The burden of this disease is estimated to 150 million cases and 1 million deaths per year. No efficient shigella vaccine is currently available. Protective immunity is conferred by a humoral response, however the cellular response and its contribution to protection remains elusive. We, therefore, investigated the shigella-induced T cell immunity using a murine model of infection and found that s. flexneri strongly induces IL-17 producing CD4+. IL-17 producing CD4+ cells (Th17) were very recently described as a separate lineage; nevertheless their function in protective immunity against pathogens was poorly defined. Shigella-specific Th17 could be found up to seven months after the clearance of infection indicating that these CD4+ cell subtype could acquire a memory-phenotype. Finally, our experiments revealed that IL-17 had an important function in the clearance of a primary and, more importantly, secondary infection. These results would contribute to the improvement of rational design of new vaccine strategies.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Shigellosis is one of the most serious cause of diarrheal disease in the world. The global impact has been estimated at over 160 million cases per year. Shigellosis is a major cause of infant mortality in developing countries with nearly 1 million deaths p er year.Shigella infiltrate and disrupt the integrity of the intestinal epithelium, initiate an inflammatory cascade, and induces apoptosis in host phagocytes. Adaptive immunity to Shigella infection is characterised by the induction of a humoral response. The duration of protection against re-infection is limited. The cellular immune response remains poorly understood. However, several data suggest that Shigella is able to manipulate the immune system to avoid the induction of a T-cell response. The specif ic aims of this project are to evaluate the CD4+ and CD8+ T-cell response during S. flexneri infection and to test the hypothesis that S. flexneri escape T-cell recognition by inducing apoptosis in T-lymphocytes.The first part includes a general analysis o f the T-cell response. The analysis will include mucosal T-cells and non-mucosal T-cells and will be performed in T-cells of the effector phase and the memory phase. Important aspects of the analysis are the induction, regulation, phenotype, and cytokine p rofile of Shigella-specific T-lymphocytes. Further experiments will investigate the T-cell dependence of protection against re-infection in different animal models.The second part of the project addresses the question whether S. flexneri induces T-cell kil ling by apoptosis. The specific aims are to analyse the effectiveness, the time-kinetics, and the possible mechanism of T-cell apoptosis induction. Strategies will be developed to test the hypothesis that T-lymphocytes apoptosis upon Shigella infection res ults in immune evasion leading to limited immunity to Shigella.
Оригинален текст от CORDIS (на английски).
Участници
- INSTITUT PASTEUR · PARISКоординаторФранция
Връзки
Данни: CORDIS, © Европейски съюз