FOPRAPRA · Food-Processing approaches to reduce allergenic potential of proteins
6РП — Действия „Мария Кюри“
- Период
- 2005-06-01 → 2006-05-31
- Финансиране от ЕС
- 40 000 €
- Участници
- 1
- Схема
- ERG
Линиите свързват координатора с партньорите.
Накратко на български
Алергенните протеини в ядките и соята се анализират, за да се разбере как се променят при храносмилането или чрез добавяне на захари. Това помага за откриването на начини за намаляване на алергичния потенциал на храните.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - FOPRAPRA (Food-Processing approaches to reduce allergenic potential of proteins)
In this project, we have been able to achieve the following objectives: i) Isolation, purification and characterisation of food allergens belonging to the 2S albumin family contained in tree nuts such as almonds (Prunus dulcis) and walnuts (Juglans regia). To accomplish this approach, we have optimised several chromatographic methods including size-exclusion, anion-exchange and reverse-phase chromatography. The identification of the different 2S albumin isomers was performed by MALDI-MS. The allergen-icity of plant food proteins may be due to a sum of the amount ingested, their stability to the processing and the gastro-intestinal tract environment, as well as their intrinsic allergenic properties. Therefore, any investigation of the structure-function relationships of allergens requires the purification, characterisation and elucidation of the structure and conformation of allergens. ii) The study of the thermostability, in vitro gastrointestinal digestibility and subsequent absorption across human intestinal epithelial Caco-2 cell monolayers of 2S albumin allergens. Our findings revealed that 2S albumin allergens may be able to survive in immunologically reactive forms to the simulated harsh conditions of the gastrointestinal tract in order to be transported across the Caco-2 cell monolayers, so that they would be able to sensitise the mucosal immune system and / or elicit an allergic response. iii) The antigenicity of soy proteins isolate was reduced by conjugation with carbohydrates such as fructose and fructo-oligosaccharides via the Maillard reaction. These results open up the possibility to decrease the allergenic potential of food proteins by interaction with other food ingredients.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Incidence of food allergy, including tree nuts, is rapidly increasing, and is a major food safety concern. Among tree nuts, almonds and walnuts are widely consumed in Spain; together with peanuts and hazelnuts they are the most commonly-cited allergen sour ces. However, to date, very few studies on altering the allergenicity of almond/walnut proteins have been performed in comparison with those on peanut/hazelnut.FOPRAPRA is a novel multidisciplinary, trans-sectorial project to examine the molecular basis of changes in the allergenic activity of proteins from walnuts and almonds [the latter being grown in Spain for export and home use] and determine means for their reduction. It is proposed by a candidate with broad experience in macromolecular chemistry and analysis and food allergy, who will work at a leading Spanish food research centre.The primary objective is gain information on the effect of processing and the nature/extent of component interactions on the structure/subsequent digestion of walnut and alm ond proteins. The effects of [1] thermal and novel processing (e.g. high pressure) and [2] carbohydrate interactions on allergen structures and their IgE-reactivity will be examined, in collaboration with clinical researchers from Madrid. State-of-the-art biochemical and spectroscopic methods will be used for structural studies and characterising carbohydrate-allergen interactions; advanced mass spectrometry techniques will be used to study allergen digestion. A Reintegration Grant would provide an ideal op portunity to transfer scientific knowledge, contacts and experience acquired during the two-year FP5 Marie Curie Grant in the UK to the experienced host institution in Spain. This will facilitate the professional reintegration, offer a basis for further re search funding, for training and networking at national/European level, and for development of durable trans-sectorial co-operations with national/European partners, including clinicians and industry.
Оригинален текст от CORDIS (на английски).
Участници
- CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS · MADRIDКоординаторИспания
Връзки
Данни: CORDIS, © Европейски съюз