NOVODISC · Development of injectable materials for intervertebral disc repair.
6РП — Действия „Мария Кюри“
- Период
- 2006-01-01 → 2007-12-31
- Финансиране от ЕС
- 223 657 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите.
Накратко на български
Инжектируеми синтетични хидрогели се тестват като заместител на увреденото ядро на междупрешленните дискове. Тези материали помагат за подобряване на терапията при хора с болки в кръста, като имитират хидратацията и поведението на естествената тъкан.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - NOVODISC (Development of injectable materials for intervertebral disc repair)
Debilitating low back pain as a result of symptomatic lumbar disc degeneration places a significant burden on an industrial society. Surgical treatments involving discectomy followed by fusion of the anterior and posterior spinal elements often result in varied and unpredictable outcomes. At present, there is much interest in using synthetic hydrogels as an alternative therapy for intervertebral implants. Currently employed synthetic hydrogels have proved to be very effective soft tissue biomaterials for a range of applications, but fail to match the behaviour of natural nucleus pulposus in terms of hydration and osmotic responsiveness. In this study, we have pursued a biomimetic approach employing the sulphonate group as a surrogate for the naturally occurring sulphate in proteoglycans to produce an injectable, in-situ polymerized, osmotically-responsive and non-degradable nucleus pulposus replacement. Thus we make use of two available precursor monomers: the sodium salt of 2-acrylamido 2, 2 methylpropane sulphonic acid (NaAMPS) and the potassium salt of 2-sulphopropyl acrylate (KSPA) using various compositions. We used an in-house artificially-induced degenerate explant model by injecting enzyme mixture to allow for proteoglycan degeneration prior to the implantation with the PG-analogue. The tailored implant injected matches the behaviour of natural tissue is terms of hydration and ionic responsiveness. It is shown to be biocompatible, as disc and fibroblast cells show no adverse cellular responses to either the monomers or the polymer itself over the concentration ranges which were utilised in this project. To address issues of visibility, a radio-opaque dye was included in the monomer mixture which appeared not to change the rheological properties. Preliminary biomechanical tests showed stability of the implant within the nucleus pulposus cavity. Using this approach, we aim to demonstrate the potential for a novel, biomimetic and minimally invasive treatment for a rapid repair and replacement of damaged intervertebral disc in cases of competent annulus fibrosus, greatly reducing the risk of a spinal surgery.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The project described in this application is concerned with developing a minimally invasive method of repairing degenerate intervertebral discs. The intervertebral discs have a mechanical function; they are the joints of the spine and support the high lo ads arising from body weight and muscle activity; maintaining high water content is essential for their function. The ability of the discs to remain hydrated under load is determined principally by the large proteoglycan aggrecan which generates a very hi gh osmotic pressure on account of its charge properties. Discs degenerate early; aggrecan is degraded and lost from the central region of the disc which thus loses its load-bearing capacity. Degenerate intervertebral discs are strongly associated with b ack pain; severe back pain affects a significant proportion of the population and is one of the most expensive of chronic disorders in western societies. At present the only treatments on offer are palliative or surgical; surgery is expensive and invasiv e with long recovery times. Here we propose an alternative method of repairing discs using new synthetic biomaterials based on charged hydrogels already in use for treatment of corneal-defects. The novelty of the proposal is that (a) treatment to introd uce the synthetic biomaterial into the disc will be only minimally invasive and so costly and expensive surgery will be avoided (b) the biomaterial will polymerise in situ, be non-toxic and bond to the healthy surrounding discs (c) the biomaterial will be engineered to mimic the physico-chemical behaviour of healthy disc tissue and so will restore disc biomechanical function. The work will involve (i) characterising factors regulating aggrecan osmotic and hydraulic permeability properties using state-of-a rt techniques (ii) manipulating charge density, chain length and branching of hydrogels to mimic relevant aggrecan properties (iii) in-situ polymerization of the hydrogel monomers in disc explants.
Оригинален текст от CORDIS (на английски).
Участници
- THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD · OXFORDКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз