XPRIMING AND TMEMORY · Study of the generation of CD8 memory T cells against soluble proteins: A step toward a new vaccine design?
6РП — Действия „Мария Кюри“
- Период
- 2006-02-01 → 2010-07-31
- Финансиране от ЕС
- 1 261 940 €
- Участници
- 1
- Схема
- SCF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
CD8+ Т-клетките на паметта се сравняват, когато са създадени чрез ваксинация срещу разтворими протеини, спрямо тези, възникнали при реална инфекция. Резултатите помагат за разработването на по-ефективни ваксини, които да осигуряват дълготрайна защита на организма.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - XPRIMING AND TMEMORY (Study of the generation of CD8 memory T cells against soluble proteins: A step toward a new vaccine design?)
The main goal of this project was to compare CD8+ memory T cells generated by cross-priming to those generated by infection. We have studied immune responses generated after vaccination in the presence of IFNa or by mimicking CD4 T cell help and compared them to responses induced by bacterial or viral infection. We have shown that vaccination generating CD8 T cells by cross priming leads to the generation of efficient memory cells able to protect the hosts. We found no significance differences with CD8 memory T cells generated by infection. Therefore, cross priming could be considered as an efficient mean to generate protective memory in vivo. We also studied the impact of the tissue origin of the dendritic cells and/or the type of signal present during activation of the T cells and showed that nor the tissue origin of the antigen presenting cells nor the type of signal present had any impact on the development of memory cells. This findings strongly suggest that memory cells are flexible and that regardless of the condition of priming they distribute all over the body and acquire both central and effector memory phenotype.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The presence of long-lasting CD8 T cell memory is crucial for protection against re-infection by many pathogens. For this reason, vaccination must aim to generate CD8 memory T cells. However, at present the generation of such cells is only achieved by vacc ination with live vaccines. These vaccines utilize attenuated viral strains which can be very difficult to generate and which can present safety hazards. Therefore, it is important to identify new ways of making safe vaccines able to generate strong CD8 me mory cells against soluble proteins. We have previously shown that we can generate strong primary CD8 responses against soluble proteins by co-administrating a natural adjuvant, IFN-a/b. These responses are generated by cross-priming and are independent of CD40. However before this approach can be applied to the development of new vaccines, some fundamental questions must be addressed. The aim of this project is to address the most important of these questions:1)To characterize, in depth CD8 memory cells ge nerated against soluble proteins by cross-priming compared to those generated after viral or bacterial infection against the same antigen2) To study the effect of antigen localisation on the development of memory CD8 T cells. The effect of the route of imm unization on the development of subsequent memory CD8 T cells will be evaluated.3) To identify the subsets as well as the activation status of dendritic cells required for the generation of long-lasting CTL responses.4) To study the effect of the tissue-or igin of the DC in the imprinting of the memory cells All together, this project should allow a better understanding of how to generate potent memory cells by cross-priming as well as providing some insights of the conditions in which dendritic cells can al low the generation of memory cells.
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERSITE RENE DESCARTES PARIS V · PARISКоординаторНиво градФранция
Връзки
Данни: CORDIS, © Европейски съюз