MAFOSE · Mannoproteins in food security
6РП — Действия „Мария Кюри“
- Период
- 2005-03-18 → 2006-03-17
- Финансиране от ЕС
- 40 000 €
- Участници
- 1
- Схема
- ERG
Линиите свързват координатора с партньорите.
Накратко на български
Манопротеините от дрожди се изследват, за да се установи кой метод на извличане най-ефективно пречи на бактериите Campylobacter да се закрепят в червата на пилетата. Това помага за подобряване на безопасността на храните и разбирането на действието на тези протеини.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - MAFOSE (Mannoproteins in food security)
Different methods for extraction of mannoproteins to use in food security to avoid Campylobacter adherence to epithelial cells of chicken gut have been studied. We had found significant differences among the methods assayed, observing that they could be determinant in the characteristics of the fractions obtained. We had prepared mannoprotein fractions using physical, chemical and enzymatic methods for mannoprotein extraction. From all the methods assayed, the high yield has been obtained with the employ of enzymatic methods. An extraction protocol has been set- up which permit the production of different mannoproteins fraction to be assayed with Campylobacter. In addition, others analytical and biological procedures has been set up during the first year of the project, related with the extraction, fractionation and characterisation of mannoproteins. Others are mainly related with the biological assays (Campylobacter adherence test) and will be very useful in the second year of the project. Also, we have studied the influence of different enological conditions in the mannoprotein fractions. We have demonstrated that fermentation stage, culture conditions and some stresses as temperature shock and ethanol stress could modify significantly the composition of the mannoprotein fractions obtained from yeast. The current project is providing novel information related to the influence of extraction methods in mannoprotein composition and characteristics. Information related to the better conditions to obtain mannoprotein fractions effective to prevent Campylobacter adherence to chicken gut tissue will be provided at the end of this project. Findings derived of this project will contribute to understand the relationship between mannoproteins composition and its potential effect on food security. These investigations may provide theoretical and practical valuable results useful for academic and food industry.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Yeast mannoprotein could be useful to reduce colonisation by enteropathogenic bacteria. The use of yeast cells or yeast cell wall preparations has been associated with the reduction of different enteric pathogens. Campylobacter je/uni and related species a re now recognised as the most common causes of bacterial foodborne diarrhoeal disease throughout the world and poultry, especially chickens, are the main reservoir of this pathogen. However, no attempts have been made to establish a relationship between specific mannoproteins and its efficacy to reduce Campylobacter in chickens.We propose as main aim of this investigation to study the effect of mannoproteins on Campylobacter adherence to ephitelial cells of chicken gut, identifying fractions especially effective against Campylobacter. Mannoprotein properties may be affected by the procedure used for its extraction and purification. Thus, different methods for mannoprotein extraction and fractionation will be evaluated. Also, some enological variables that ma y have influence on the mannoproteins obtained, as the different steps of winemaking, culture conditions and relevant enological stresses, will be studied.Mannoproteins as inhibitor of Campylobacter adherence will be tested, trying to establish a relation ship between mannoproteins composition and its capacity to inhibit Campylobacter adherence in vitro. This information may contribute to establish novel industrial process for preparation of mannoproteins and may have a positive influence on the selection o f new natural strains and the genetic engineering experiments designed to obtain super producers strains of specific mannoproteins. Findings derived of this project will contribute to understand the relationship between mannoproteins composition and its potential effect on food security. These investigations may provide theoretical and practical valuable results useful for academic and food industry.
Оригинален текст от CORDIS (на английски).
Участници
- CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS · MADRIDКоординаторИспания
Връзки
Данни: CORDIS, © Европейски съюз