PLANT COLD TOLERANCE · A novel non-CBF-regulon involved in cold acclimation in Arabidopsis
6РП — Действия „Мария Кюри“
- Период
- 2005-01-01 → 2007-12-31
- Финансиране от ЕС
- 274 055 €
- Участници
- 2
- Схема
- OIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Генетичният път за създаване на глюкорафанин е пренесен в тютюнево растение, за да се разберат гените, които го контролират. Това помага за повишаване на съдържанието на вещества с противоракови свойства в зеленчуците.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - PLANT COLD TOLERANCE (A novel non-CBF-regulon involved in cold acclimation in Arabidopsis)
Glucosinolates are natural plant products with many biological functions, such as defence compounds and flavour compounds in human consumption. Glucosinolates are degraded by endogenous myrosinases, or by microbial organisms in the gut. Certain glucosinolates are degraded into potent cancer-preventive agents such as sulphoraphane, which is derived from the glucosinolate glucoraphanin. The cancer-preventive properties of sulphoraphane are well documented, and there is a great interest in improving the glucoraphanin content in vegetables. Glucoraphanin is derived from the amino acid methionine. Through a three-step reaction, methionine is converted first to Dihomo-methionine (DHM), then to a parent glucosinolate called 4-methylthiobutyl glucosinolate, and finally to glucoraphanin. During this project, we have introduced the entire glucoraphanin biosynthetic pathway into the tobacco plant, Nicotiana benthamiana and in the process elucidated the pathway and identified several key genes. We have shown reconstitution of DHM biosynthesis and in the process discovered three new genes that play key roles in this pathway. We have coupled DHM biosynthesis to glucosinolate biosynthesis and have succeeded in modifying the parent glucosinolate produced to the cancer-preventive glucoraphanin. In addition, we have assigned function to several previously uncharacterised genes in this part of the pathway. The ability to transfer an entire 13-gene biosynthetic pathway to a heterologous organism is unprecedented and opens up vast opportunities for producing natural products in other species. 4-MSB has been found in a limited number of species in only five families of the Brassicales order. Due to its potent cancer-preventive properties, there is a great interest in investigating if it was possibly to reconstitute this pathway in other species. However, other glucosinolates are potent cancer-preventive agents such as 7-MSH and 8-MSO. These glucosinolates could be produced using the current method with few modifications. The current method can be used to produce any desired glucosinolate for use as flavour compounds, bio-medicine, bio-pesticides, anti-microbial agents, as additives in functional foods or in cancer treatment, for production in planta or in micro-organisms.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Cold acclimation allows certain plants to increase their cold tolerance cold upon exposure to low non-freezing temperatures. Improving cold tolerance of crop plants is of great importance as frost damage annually amounts to about 14 billion Euro worldwide. The 'high-yield' crops produced during the 'green revolution' were results of altering transcription factor (TF) activities. Biotechnological approaches aimed at altering TF activities presents an attractable solution for improving complex traits such as cold tolerance, yield, pest resistance, bio-flavour, and nutritional qualities.The group of Prof. MF Thomashow at Michigan State University (MSU) represents the world-leading centre for plant cold acclimation. My objectives are to learn the-state-of-the-art techniques within micro-array analysis, data mining and bioinformatics, DNA-protein and protein-protein interaction screens. I intend to improve my managerial competences in specific programs designed by the MSU HR. Almost 1000 genes are co-ordinately regulated in response to cold.The project will focus on one group of co-ordinately regulated cold-responsive genes, a regulon. Expression levels of regulon-specific cold-responsive TFs will be altered in planta for plant cold tolerance and transcriptome analyzes. DNA-protein and protein-protein screens will be used to identify regulatory components that are not transcriptionally controlled. The state-of-the-art techniques acquired during the outgoing phase will be implemented at the reintegration institution i n a directed mutant screen that I have setup. Furthermore, this expertise will be implemented at the MSU for mutual benefit and for establishing long-term collaborations.The mobility of the current project provides unique possibilities for acquiring emergent new technologies and for improving my managerial and leadership competences. It will help me develop independence as a researcher and to found my own pioneering group.
Оригинален текст от CORDIS (на английски).
Участници
- THE ROYAL AGRICULTURAL AND VETERINARY UNIVERSITY · FREDERIKSBERG CКоординаторНиво държаваДания
- MICHIGAN STATE UNIVERSITY · EAST LANSINGСъединени щати
Връзки
Данни: CORDIS, © Европейски съюз