METCLEF · Novel metal clusters in protein clefts
6РП — Действия „Мария Кюри“
- Период
- 2008-09-01 → 2009-08-31
- Финансиране от ЕС
- 89 100 €
- Участници
- 1
- Схема
- IIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Протеинът трансферин и начинът, по който той образува нишки и отделя железни кристали, се анализират чрез микроскопия. Разбирането на този механизъм помага при разработването на лекарства срещу невродегенеративни заболявания, свързани с натрупването на желязо в мозъка.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - METCLEF (Novel metal clusters in protein clefts)
We were the first to observe that human serum transferrin could form fibrils. This 80 kDa bilobal protein transported iron (Fe3+), tightly bound to four amino acid side-chains and carbonate as a synergistic anion in closed interdomain clefts, one in each lobe. We showed that dehydration of iron transferrin on surfaces readily led to dendritic growth, fibril formation and release of iron which formed nanocrystallites of iron (hyd)oxide, i.e. rust, at periodic intervals along the length of the fibres. These findings might shed new light on iron deposition, which was thought to be involved in the pathogenesis of various neurodegenerative diseases. Furthermore, such reactions promoted the nanocrystallisation of manganese, which was also an essential metal, and bismuth, a metal used in therapy. In addition, we studied the behaviour of transferrin on surfaces using various microscopic techniques, which was never done previously. We observed the periodic nanocrystallisation of iron (hyd)oxide (rust) in a novel process involving dendritic outgrowths of the protein. Such periodic nanocrystallisation had not been previously observed. Neurodegenerative diseases such as Parkinson, Alzheimer and Huntington diseases and Hallervorden-Spatz syndrome were associated with the misregulation of iron regulation in the brain as well as with iron deposition, even though the causes were not firmly established. In case transferrin played a role in accumulation of iron in brain tissues, understanding the mechanism of this process would allow for the design of drugs which could disrupt transferrin aggregation and be used for treatment purposes.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The assembly, structure, and properties of novel iron and other metal nano-clusters in the cleft of the protein bacterial transferring will be investigated. The work is of high significance for understanding iron uptake by pathogenic bacteria and the mechanisms of bio-mineralization. Small protein-bound oxo iron clusters are of potential importance in the design of novel materials with unusual magnetic and catalytic properties, and of novel antibiotics. This bioinorganic chemistry project will be highly interdisciplinary, crossing the boundaries of inorganic chemistry, biology, biophysics, medicine and materials science.The host institution is well equipped with state-of-the-art equipment and facilities for the proposed project either directly within the School of Chemistry in Edinburgh or in associated centres, e.g. Edinburgh Protein Interaction Centre (EPIC), Institute for Cell and Molecular Biology (ICMB), Scottish Centre for Genomic Technology and Informatics (GTI), Collaborative Optical Spectroscopy and Micromanipulation Centre (COSMIC), Institute for Integrated Micro and Nano Systems (IMNS), Centre for Science at Extreme Conditions (CSEC), and the Interdisciplinary Research Centre in Proteomic Technology (Radical Solutions for Researching the Proteome, RASOR). Mobility to the host country under the supervision of Professor Sadler and visits to the laboratories of European collaborators will provide the applicant with unique training for an independent future career in academia or industry.The applicant will learn to establish a programme as an independent research worker, acquire new cutting-edge skills, participate in multicultural research teams, experience the benefits of cultural diversity and acquire an international profile for his career. The project will have a significant impact on scientific and technological links between Europe and India, in particular with the Indian Institute of Technology in Kanpur, the host for the reintegration year.
Оригинален текст от CORDIS (на английски).
Участници
- INDIAN INSTITUTE OF TECHNOLOGY KANPUR · KANPUR, UTTAR PRADESHКоординаторНиво държаваИндия
Връзки
Данни: CORDIS, © Европейски съюз