NICKEL RHINITIS · Functional characteristics of allergen-specific T lymphocytes in nickel-induced allergic rhinitis
6РП — Действия „Мария Кюри“
- Период
- 2005-05-16 → 2006-05-15
- Финансиране от ЕС
- 40 000 €
- Участници
- 1
- Схема
- ERG
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Алергичните реакции към никел се анализират чрез изследване на специфични Т-лимфоцити и цитокини при пациенти с кожен обрив или ринит. Това помага да се разбере защо един човек развива кожни възпаления, а друг – симптоми в носната лигавица.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - NICKEL RHINITIS (Functional characteristics of allergen-specific T lymphocytes in nickel-induced allergic rhinitis)
The aim of the present study was to find correlates between phenomena observed in vitro, and the clinical symptoms of nickel allergy. Additionally, we attempted to explain the factors determining that one person will react with skin inflammation, whereas another one - with rhinitis. In 40 persons with allergic contact dermatitis to nickel (Ni-ACD), we sought potential correlations between nickel-specific cytokine secretion and the outcome of patch test in the group of patients with allergic contact dermatitis to nickel. The levels of IL-5 and IFN-gamma secreted by peripheral blood lymphocytes (PBMC) in response to nickel measured with ELISA were correlated with the intensity of skin inflammation (patch test score). Additionally 19 patients with nickel-related rhinitis (Ni-Rh) and 22 patients with Ni-ACD, and 17 non-allergic volunteers were studied. The percentage of nickel-reactive cells was assessed in cells expressing CLA (skin-related homing antigen) and cells expressing HML/CD107 (mucosa-related homing antigens). Ni-specific secretion of IL-2 (naive cells), IL-13 (Th2/Tc2), and IFN-gamma (Th1/Tc1) was measured by means of ELISPOT (enzyme-linked immunospot assay). CD14+ monocytes were used as antigen presenting-cells (APC). We have demonstrated that nickel-specific IL-5 secretion by PBMC determines the intensity of patch test reaction (p=0.05), with no significant effect of IFN-gamma. An increase in the signal of IL-5 by 10 pg/ml is associated with a 24.2% increase in the predicted odds of patient being in upper category of patch test score (p=0.05). IFN-gamma had no significant influence on the odds ratio. An increase in the nickel-specific IL-5 secretion by 10 pg/ml is associated with a 10-20% increases (depending on statistical model) in the odds ratio of the patient to have a higher patch test score. This supports the assumption that cells secreting IL-5 (e.g. Th2, Tc2) play more important role in the pathogenesis of ACD than previously thought. No clear differences could be found between the cytokine secretion between the lymphocyte subpopulations (CLA+, HML-1+), and between the studied groups (patients with nickel rhinitis, Ni-ACD patients, healthy controls). We have demonstrated that the level of the allergen-specific IL-5 production is a relevant predictor of patch test score, whereas IFN-gamma is not. This supports the assumption that type 2 cells secreting IL-5 (e.g. Th2, Tc2, possibly also NK2 or NKT2) play an important (if not crucial) role in the pathogenesis of allergic contact dermatitis. We were not able to demonstrate a different cytokine response to nickel of lymphocyte subsets with different homing antigens.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Allergy to nickel is a frequent health problem that affects as many as 60 million people in Europe and poses a considerable burden to the EU economy. In order to gain a better insight into the nature of the disease, this laboratory-based project will be carried out on nickel-specific T-lymphocytes ¿ cells playing a key role in initiating the disease.Particular attention will be paid to differences between lymphocytes involved in two different clinical forms of nickel allergy: nickel-induced allergic rhinit is (Ni-AR) and nickel-induced allergic contact dermatitis (Ni-ACD). The working hypothesis is that these lymphocytes express different homing antigens and/or chemokine receptors that allow them to migrate and initiate inflammatory processes in particular organs, i.e. in the nasal mucosa or the skin.In order to verify this hypothesis, lymphocytes of patients with Ni-AR, Ni-ACD, and healthy controls will be studied for their functional characteristics (cytokine secretion) and the presence of molecules involved in lymphocyte homing. This project is an extension of the research carried out within the applicant's current Marie Curie Individual Fellowship and will enable him to transfer the newly acquired knowledge and laboratory techniques to his country of origin.The reintegration host has a long experience in nickel research and provides health care to many patients with Ni-AR and Ni-ACD, which will provide a good opportunity for recruiting volunteers to donate blood samples needed for this study.The expected results will provide a better understanding of the mechanisms of nickel allergy and will be a first step towards the development of an in vitro method for the diagnosis of nickel rhinitis.
Оригинален текст от CORDIS (на английски).
Участници
- JAGIELLONIAN UNIVERSITY MEDICAL COLLEGE · KRAKOWКоординаторНиво градПолша
Връзки
Данни: CORDIS, © Европейски съюз