GNRH SYSTEMS IN COD · Characterization of GnRH systems in Atlantic cod: cloning, brain distribution, and mechanisms of action
6РП — Действия „Мария Кюри“
- Период
- 2005-07-20 → 2006-07-19
- Финансиране от ЕС
- 40 000 €
- Участници
- 1
- Схема
- ERG
Линиите свързват координатора с партньорите.
Накратко на български
Системите от хормони (GnRH) в мозъка на атлантическата треска регулират процесите по достигане на полова зрялост. Тези анализи помагат за по-доброто разбиране на развитието на рибите от групата на костилистите.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - GNRH SYSTEMS IN COD (Characterization of GnRH systems in Atlantic cod: cloning, brain distribution, and mechanisms of action)
Puberty in fish, as in all vertebrates, is regulated mainly through the brain-pituitary-gonad axis. In this ERG project, we have focused on the brain-pituitary axis of Atlantic cod, characterizing the stimulatory gonadotropin-releasing hormone (GnRH) systems in the cod brain and pituitary. We have characterised two cDNAs encoding two forms of preproGnRH in the Atlantic cod, sGnRH and cGnRH-II. Furthermore, we have characterised four cDNAs encoding four distinct GnRH-Rs (type 1b, 2a, 2b, and 2c, respectively). Phylogenetic analyses of the isolated genes confirm their identity with the homologous genes of other teleost fish. This molecular characterisation provides the molecular basis for our long-term research programme: development of specific riboprobes for in situ hybridisation experiments to investigate the cellular distribution of the cloned genes, development of specific primers for quantitative real-time RT-PCR assays, to monitor quantitatively the gene expression during pubertal development and following treatment with sexual steroids and manipulation of photoperiod. The impact of the identified GnRH ligands on gonadotropes in cod will also be investigated using electrophysiological (patch-clamp) techniques. Together, these investigations will contribute to a better understanding of pubertal development in teleost fish, and the Atlantic cod in particular.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Early sexual maturation leads to a significant loss of body weight during the spawning season, and farmed cod of both sexes currently reach puberty as early as two years of age, before reaching commercial size.However, development of effective treatments to delay puberty and control spawning is hampered by the lack of basic knowledge on the regulatory mechanisms controlling pubertal development in cod, most notably the neuroendocrine control where no information is available.The main objective with this project is to characterize the different GnRH systems in the brain and pituitary of Atlantic cod, and to investigate how these are regulated. This will be done by cloning and sequencing of the different GnRH forms and their receptors, monitoring the cellular distribution and expression profiles of GnRH and their receptors in the brain and pituitary throughout pubertal development.Furthermore, to analyze the feedback mechanisms participating in the activation of the brain-pituitary-gonad axis, we will investigate how the GnRH and GnRH receptors are regulated by steroid hormone treatments. Also, a close collaborating scientist will simultaneously investigate by electrophysiological methods the cellular mechanisms involved in basal and GnRH stimulated activity of cod gonadotropes.In addition to providing new insights into the GnRH systems of a representative from the poorly studied order of gadiformes (Teleostei), this project should contribute to an improved understanding of the neuroendocrine regulation of pituitary function in teleost fish in general.
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERSITETET I OSLO · OSLOКоординаторНорвегия
Връзки
Данни: CORDIS, © Европейски съюз