FISHBONEP · Role of Parathyroid Hormone related Protein (PTHrP) in endochondral bone development: Application as a possible marker for skeletal anomalies in fish
6РП — Действия „Мария Кюри“
- Период
- 2005-11-01 → 2007-04-30
- Финансиране от ЕС
- 126 261 €
- Участници
- 2
- Схема
- OIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Ролята на протеина остеонектин при развитието на скелета се проучва чрез наблюдение на ембриони на зебра риба. Това помага да се разбере как се формират хрущялите и какви дефекти възникват при липса на този протеин.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - FISHBONEP (Role of Parathyroid Hormone related Protein (PTHrP) in endochondral bone development: Application as a possible marker for skeletal anomalies in fish)
The following is a brief summary of the completed studies on the role of osteonectin in endochondral bone development. Osteonectin is a matricellular glycoprotein expressed in many differentiated cells which is critical for organ development and cell processes such as cell turnover, remodelling and tissue repair. Osteonectin, well conserved in evolution, is a major non-collagenous constituent of vertebrate bones. Growing bodies of evidences indicate that ostonectin could play a role in cell differentiation, particularly in chondrocytes. However, a precise role for osteonectin and its regulation in chondrogenesis is known. We therefore used a morpholino-based knockdown approach to investigate the functions of osteonectin. During zebrafish embryo development, osteonectin was expressed in the notochord, floor plate, esclerotome, otic vesicle, eye and epidermal fin folds. However, its expression was tightly regulated in cartilage structures. The morphant embryos showed reductions in Meckel's cartilage, small kinked ceratohyals and shorter pectoral fins. In morpholino-injected animals the expression of col2a1 was strongly decreased but did not influence sox9 expression. Moreover, we found that osteonectin expression was down-regulated in sox9 mutant embryos. % In addition, knockdown of osteonectin led to defective semicircular channels without affecting the otoliths. As a consequence, the morphant larvae failed to balance. These phenotypes were osteonectin-specific because osteonectin messenger Ribonucleic acid (mRNA) lacking morpholino recognising site rescued morphant phenotypes. Thus, this study provided the first characterisation of the involvement of osteonectin in chondrocyte differentiation, showed that osteonectin acted downstream of sox9 in a cascade of genes' regulation and indicated that osteonectin was a possible modulator of chondrogenesis.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The high incidence of larvae skeletal deformities is a significant problem in European marine aquaculture. This problem has a serious impact on the industry's productivity, on product quality and on animal welfare. The factors, which may cause skeletal anomalies in fish are largely unstudied, although in tetrapods there is ample evidence that PTHrP plays a significant role in both normal and abnormal development and growth of the skeleton. Preliminary studies carried out by the CME group indicate that PTHrP may be implicated in skeletal deformities, which arise as a consequence of altered chondrogenesis and calcification.Thus, the fundamental scientific objective of this project is to investigate the importance of the unique hypercalcemic factor identified in fish, PTHrP, in endochondral bone development and its application as a possible marker for skeletal anomalies in fish. This will be achieved through the application of recombinant DNA and gene transfer technology as a tool for the induction of PTHrP gene over expression in fish embryos. The present project programme will therefore aim to establish unequivocally the role of PTHrP in chondrogenesis and skeletal development and link this to skeletal abnormalities. Several objectives have been identified which will contribute to the overall achievement of the aim.Firstly, obtention of PTHrP expression construct and its microinjection into fertilized fish eggs; Secondly, assessment the ectopic expression of PTHrP in notochord cells of fish embryos and finally, evaluation the influence of PTHrP in the development of axial skeleton. Moreover, additional objectives of this research proposal are to promote the scientific development of the applicant and transfer the technology and knowledge acquired at the host institute during the visit to USA to the CME group at the return host institute. This will be important for the development of these groups' objectives but will also benefit the whole academic community.
Оригинален текст от CORDIS (на английски).
Участници
- CENTRO DE CIENCIAS DO MAR DO ALGARVE · FAROКоординаторПортугалия
- CENTER OF MARINE BIOTECHNOLOGY UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE · BALTIMORE, MARYLAND (MD)Ниво държаваСъединени щати
Връзки
Данни: CORDIS, © Европейски съюз