SUNKEN WOOD MICRODIV · Biodiversity and functions of microbial communities associated with sunken woods in marine environments
6РП — Действия „Мария Кюри“
- Период
- 2005-06-01 → 2007-05-31
- Финансиране от ЕС
- 214 590 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Микробите, живеещи върху потънали дървета в крайбрежни и дълбоководни зони, се анализират чрез генетични методи и електронна микроскопия. Това помага да се разбере как органичната материя влияе върху биоразнообразието и еволюцията на общностите в дълбините на океана.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - SUNKEN WOOD MICRODIV (Biodiversity and functions of microbial communities associated with sunken woods in marine environments)
Different sources of imported, organic matter carried down to the deepest parts of all seas could enhance the species' richness of sediment-dwelling communities where organisms are highly food-limited and rely primarily on organic material from upper layers. Furthermore, when deposited in marine sediments, large organic falls undergo sufficiently steady decay for oxygen to be depleted, attracting anaerobic living forms. The phylogenetic resemblance and overlap in species of metazoans living in woods or large animal remains with those of highly reduced environments like hydrothermal vents and cold seeps has lead to the hypothesis that deep-sea organic rich matter deposits could play a major role in the dispersion and evolution of chemoautotrophic communities at the ocean basin. Chemosynthetic symbiotic and free-living bacteria drive these communities. No culture-independent studies on free-living microorganisms at sunken woods have been conducted so far. We used culture-independent methods to study the microbial communities on both artificially immersed and naturally sunken wood logs in geographically distant coastal and deep-sea environments. We used a fingerprinting method called Capillary electrophoresis single stranded conformation polymorphisms (CE-SSCP) to fingerprint the archaea and bacteria communities living on and within the woods to determine their resemblance among samples. Scanning electron microscopy revealed extensive microbial erosion of the wood cell walls and different decay patterns with length of immersion. Furthermore, posterior ribosomal Ribonucleic acid (RNA) gene (rDNA) cloning and sequencing of target samples gave clues on the actual diversity and phylogenetic resemblance of the main microbial players responsible of energy and carbon flows. Our results broadened our narrow knowledge on the microbial biofilms that developed around woods in marine environments and gave clues on the ecological importance of these organic island deposits for the dispersion of chemosynthetic communities at the ocean basin.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Sunken woods are widespread in the ocean with highest densities found within sediment accumulation basins and canyons. Recently evidence is accumulating on the importance of these organic matter deposits in the ecology of the ocean because of their potential role in the overall carbon budget of the marine waters. They could also be crucial in the dispersion of organisms across the ocean basin acting as stepping stones" to other highly reduced habitats. Understanding the mechanisms governing the transformation of this pool of carbon could give clues on a more sustainable management of the ocean.Despite their importance, to the best of our knowledge there are no microbial diversity studies of wood remnants in the ocean. In the present proposal our aim is to determine the ecosystem-level role of individual microorganisms associated to sunken woods by linking microbial diversity (i.e. assemblage composition) with functions (i.e. biogeochemical role). Serial Analysis of Gene Tags (SAGT), a newly developed s mall subunit rRNA gene sequence-based methodology that can determine relative abundances of microorganisms will be use to characterize the sunken wood microbial ecological diversity.SAGT will be combined with flow cytometry to investigate active microbial consortia. Correlations of the biodiversity encountered with concomitant physicochemical measurements will help to understand the biogeochemistry of these habitats. We will likely find a shift from heterotrophic to chemosynthetically based communities a long the decomposition process, which has never been demonstrated in sunken woods. We will further characterize the functional diversity of geochemically relevant genes through phylogenetic analysis. Because microbial communities in these habitats are to tally unexplored, new species are likely to be discovered. New strains will be cultured and readily available for the industry as these microorganisms have a great potential importance for biotechnology."
Оригинален текст от CORDIS (на английски).
Участници
- CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DELEGATION LANGUEDOC-ROUSSILLON · MONTPELLIERКоординаторНиво градФранция
Връзки
Данни: CORDIS, © Европейски съюз