CELLDIV · Analysis of interactions between cell division proteins
6РП — Действия „Мария Кюри“
- Период
- 2005-07-01 → 2008-06-30
- Финансиране от ЕС
- 250 232 €
- Участници
- 2
- Схема
- OIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Протеините, които разделят бактериалната клетка на две, се анализират чрез взаимодействието им при бактерии като E. coli. Разбирането на тези молекулярни процеси помага при търсенето на нови антибактериални лекарства.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - CELLDIV (Analysis of interactions between cell division proteins)
Cell division is a fundamental process that allows for the separation of one mother cell in two identical daughter cells. This mechanism is essential for the survival of bacteria and is highly regulated in time and space by many factors. Understanding, at the molecular level, the machinery running this key process is crucial to open new opportunities of research, particularly in the antibacterial drug discovery. In this project, we were interested in protein factors recruited in the middle of the cell, which catalysed the constriction of the bacterial cell envelope to lead to the separation in two identical cells. Our aim was to understand how those central factors communicated and interacted to each other. One phase of the project was focussed on a complex composed of three essential proteins that played a central role in cell division of the gram-negative bacteria Escherichia coli. Using a combination of biochemical, genetic and cytological approaches, we made significant progress in the understanding of this complex and we proposed a model of the interactions between these three essential components and the other proteins involved in the cell division machinery. In a second phase, I exploited the cell division machinery of escherichia coli to develop a novel genetic assay for studying protein-protein interactions under in vivo conditions. In this approach, called 'E. coli artificial septal targeting', an interaction between two proteins was revealed by an easy detectable phenotype, a fluorescence signal in the middle of the cell. Using this assay, I studied the assembly of cell division proteins from the gram-positive bacteria, bacillus subtilis. This investigation helped to characterise interactions between some essential components involved in cell division and to understand the main difference between gram-positive and gram-negative bacteria cell division mechanisms. This study also demonstrated that this new genetic assay might be an efficient tool for detecting stable protein-protein interactions.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Cell division is a fundamental process that allows the multiplication of all bacteria. Although this mechanism is essential for their survival, it is one of the least understood processes in bacterial cell biology. Understanding the molecular function of different factors implicated in this key event of the bacterial cell cycle is essential. Furthermore the characterization of cell division machinery will open up new opportunities in bioengineering and development of novel therapeutic and antibacterial agents.During the past ten years, genetic approaches as well as major improvements in cytological and immunofluorescence techniques generated a wealth of data on the localization and recruitment of cell division proteins to the septum. However, specific protein interactions and complex formation have not been studied in great detail. This research project is directed at elucidating the interactions and communication between cell division proteins in Gram-negative bacteria, thus initiating a new dimension to the study of cell division.The first phase of the project will emphasize the use of biochemistry to define direct interactions between proteins assembled at the septum and to identify and characterize new cell division components. During the second phase, a n innovative and powerful genetic tool based on bacterial double hybrid system will be developed in order to validate, in an in vivo context, protein interactions and the new partners unveiled by genetic and biochemical approaches. This project will contribute to the development of a competitive high impact field in Europe by significantly extending our knowledge on cell division process and by combining classical and innovative approaches.
Оригинален текст от CORDIS (на английски).
Участници
- INSTITUT PASTEUR · PARISКоординаторФранция
- HARVARD UNIVERSITY · CAMBRIDGE, MAНиво градСъединени щати
Връзки
Данни: CORDIS, © Европейски съюз