QUMGAR · Quantitative modeling of the gal regulon
6РП — Действия „Мария Кюри“
- Период
- 2006-09-01 → 2008-08-31
- Финансиране от ЕС
- 80 000 €
- Участници
- 1
- Схема
- IRG
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Бактериите E. coli се изследват за това как интегрират различни сигнали от средата, за да регулират метаболизма на галактозата. Това помага да се разбере как клетките обработват информация и определят оптималното ниво на експресия на своите гени.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - QUMGAR (Quantitative modeling of the gal regulon)
Bacteria sense a wide array of signals (minerals, nutrients, stress signals, etc.). A large class of cellular response systems regulates the flux and concentration of small molecules by controlling their transport and metabolism. Many of the genes encoding components of such response systems are regulated by two or more signals, therefore the cell needs to integrate environmental signals and compute the optimal gene expression levels. In this work we used the galactose utilisation system of Escherichia coli to study signal integration at the level of promoter transcription. We combined the transcriptional network of the galactose regulon, obtained from our experiments, with literature data to construct an integrated map of the galactose network. Input functions (the correlation between the levels of input signals and the promoter activity) were determined by a multidisciplinary approach, combining biochemical and genetic experiments with computation. Input functions of the galactose system promoters are diverse. We observed simple shapes resembling Boolean logic gates and more complex shapes that can be approximated by the combination of different simple logic gates. The response range of input functions shows good agreement with the potential physiological range of the input signals.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Completion of genome sequencing of several model organisms has dramatic impact on science. In the post-genomic era research efforts are focused on understanding intracellular molecular interactions underlying cellular function. Functional genomic research aims to build whole-cell models of microbes and other organisms, utilizing advancements in several areas of science. The models constructed can be useful both in studying fundamental aspects of biology, in understanding disease-cells, and in engineering c ells for industrial production of biochemicals.Regulation of gene expression is a main component in cellular function. In prokaryotic organisms the primary role of transcriptional regulation is to enhance the cells performance in changing environmental conditions. I would like to use the gal regulon as a model system to enhance the understanding of the design principles, behaviour and evolution of microbial transcriptional networks. This project aims the reconstruction and quantitative modelling of the galactose regulatory network in Escherichia coli.The galactose regulon contains genes involved in the transport and metabolism of the sugar D-galactose, and genes for two transcriptional regulators. D-galactose is important for E. coli as an energy source and also as a component required for complex polysaccharide formation. The project involves experimental verification of network interactions both in vitro and in vivo, together with evaluation of their biological significance. The transcriptional units of the gal regulon are regulated by two specific regulators (GalR and GalS) and a global regulator (CRP), responding to two major input signals, D-galactose concentration and cAMP concentration, respectively. Regulation involves combinations of different mechanisms (contact inhibition/activation, steric hindrance, and DNA looping), making the gal regulon an optimal system to study integration of global and specific signals at the level of transcriptional regulation.
Оригинален текст от CORDIS (на английски).
Участници
- EÖTVÖS LORÁND UNIVERSITY · BUDAPESTКоординаторНиво градУнгария
Връзки
Данни: CORDIS, © Европейски съюз