CHARADE · Characterisation of major Ralstonia solanacearum type III pathogenicity Determinants
6РП — Действия „Мария Кюри“
- Период
- 2006-09-01 → 2007-08-31
- Финансиране от ЕС
- 40 000 €
- Участници
- 1
- Схема
- ERG
Линиите свързват координатора с партньорите.
Накратко на български
Бактерията R. solanacearum използва специална система за вкарване на протеини (ефектори от фамилията awr) в растителните клетки. Разбирането на тези механизми помага да се разбере как се развиват симптомите на увяване при растенията.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - CHARADE (CHaracterisation of major RAlstonia solanacearum type III pathogenicity DEterminants)
Most pathogenic bacteria are endowed with a Type III secretion system (TTSS) that directly translocates bacterial proteins into the host cell cytoplasm. Besides its inherent interest as a paradigmatic example of information transfer between bacteria and eukaryotes, the TTSS has been shown to be a major pathogenicity determinant. Thus, the study of bacterial effector proteins injected through the TTSS to promote disease is a question hic et nunc in plant and animal pathogenicity. This study was oriented towards a better understanding of the awr effector gene family, found in the plant pathogen R. solanacearum, whose members exhibit no similarities in the databases. The work carried out in this project included a systematic functional analysis of the awr genes and their protein products. An intriguing cell death phenotype has been observed when the genes are transiently expressed inside plant cells and some of their plant targets have been identified. Experiments are under way to unveil the exact mechanisms through which these bacterial proteins contribute to the development of plants wilting symptoms. Outcomes of this work include several presentations in workshops and congresses (two poster presentations) as well as the training of two undergraduate students and a PhD student. The interesting scientific results enable us to envision, as foreseen, the publication of an original research article in a high or medium-impact journal at the end of the second year of the project.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The discovery of type III secretion systems during the last decade has dramatically changed our understanding of the molecular basis of bacterial pathogenicity. Type III secretion systems are conserved in animal and plant bacterial pathogens and are involved in the delivery of bacterial effector proteins into the host cells. In plants, the identification of bacterial effectors is of an outstanding importance, since the understanding of the molecular processes leading to disease development and the identification of the corresponding molecular targets in the eukaryotic cell opens the way to new biotechnological strategies for crop protection.In the plant pathogen R. solanacearum biochemical and genetic approaches led to the identification of several proteins that are exported through the Type III apparatus. The advent of the genome sequencing projects has facilitated the identification of new effectors based on the similarity or the structural features of predicted proteins, as well as the coregulation with th e Type III secretion system. A group of related genes that are called the awr family and have no similarities in the databases has been identified through analysis of R. solanacearum genome sequence. Mutation of one awr gene dramatically decreases R. solana cearum aggressivity on tomato, which renders the AWR proteins excellent candidates as effector proteins that carry out a function within the plant cell.The aim of this project is to characterise thoroughly this gene family in search for type III-dependent effectors with a major role in pathogenicity. The process will include:1) demonstration of protein translocation into plant cells,2) study of their expression profile and analysis of the mutant phenotype regarding pathogenicity,3) subcellular localisati on of the AWR effector proteins once inside the plant and4) identification of the plant targets5) establishment of the role of AWR effectors and their interactors in pathogenicity or defence.
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERSITAT DE BARCELONA · BARCELONAКоординаторИспания
Връзки
Данни: CORDIS, © Европейски съюз