NERCC1_NEK6_7 · A novel mitotic signalling pathway composed by the NIMA family protein kinases Nercc1, Nek6 and Nek7. Regulation, functions, and possible implication in cellular transformation
6РП — Действия „Мария Кюри“
- Период
- 2006-02-01 → 2008-01-31
- Финансиране от ЕС
- 80 000 €
- Участници
- 1
- Схема
- IRG
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Протеиновите кинази Nercc1, Nek6 и Nek7 регулират разпределението на ДНК чрез управление на молекулярните „кабели“ и мотори в делящите се клетки. Разбирането на този механизъм помага да се изясни как функционира клетъчното делене и какви са връзките му с клетъчните трансформации.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - NERCC1_NEK6_7 (A novel mitotic signalling pathway composed by the NIMA family protein kinases Nercc1, Nek6 and Nek7. Regulation, functions, and possible implication ?)
We studied the regulation and function of the signalling module formed by the NIMA-family kinases Nercc1/Nek9, Nek6 and Nek7. Protein kinases are molecular switches used by the cell to control the function of the different molecular parts that it needs to perform its function. Nercc1, Nek6 and Nek7 are involved in the division of the cells, specifically in the separation of the mother cell's DNA into two equal groups to be inherited by the daughter cells. For this, Nercc1, Nek6 and Nek7 are supposed to control different proteins that form the mitotic spindle, a molecular structure of cables and motors that move the DNA during mitosis, the division of the cell nucleus. We have found that these protein kinases indeed bind to different proteins involved in the function of the spindle, and that they directly control one of the central spindle motors.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
It is proposed to study a novel mammalian signalling pathway composed of the NIMA-family protein kinases Nercc1/Nek9, Nek6 and Nek7. Neks are related to NIMA, an Aspergillus kinase required for mitotic entry, and are involved in chromatin condensation and formation of the spindle. Nercc1 was identified as a human Nek that binds, phosphorylates and activates two members of the same family, the homologous Nek6 and Nek7.Nercc1 is active at the centrosomes and spindle poles during mitosis, and interference with the kinase function results in spindle abnormalities and incorrect chromosome alignment, resulting in prometaphase arrest or abnormal chromosome segregation and aneuploidy. It is proposed to study the regulation of the Nercc1/Nek6/7 signalling cassette, as well as to identify substrates of these kinases and determine their role during mitosis and the formation of the spindle. I will seek to determine the mechanism that keeps Nercc1 inactive in interphase and activates it in mitosis, as well as to identify Nercc1 and Nek6/7 binding partners.The role of the kinases during mitosis will be studied through the use of different inhibiting strategies, including the production of knockout cells and mice. The goal of this proposal is to understand the position of the Nercc1/Nek6/7 pathway in the midst of different signalling pathways that control and execute mitosis, to determine whether its deregulation may be involved in cellular transformation through contribution to genetic instability, and to identify Nercc1 inhibitors and assess them as therapeutic anti-mitotic drugs.
Оригинален текст от CORDIS (на английски).
Участници
- INSTITUT DE RECERCA BIOMÈDICA DEL PARC CIENTÍFIC DE BARCELONA · BARCELONAКоординаторНиво градИспания
Връзки
Данни: CORDIS, © Европейски съюз