ASA · Alternative splicing in developmental control and stress response in Arabidopsis thaliana
6РП — Действия „Мария Кюри“
- Период
- 2006-01-01 → 2007-12-31
- Финансиране от ЕС
- 80 000 €
- Участници
- 1
- Схема
- IRG
Линиите свързват координатора с партньорите.
Накратко на български
Алтернативното сплайсване при растението Arabidopsis thaliana определя как се създават различни протеини, например чрез промяна в реакцията към захари. Процесът помага да се разбере как растенията се развиват и се адаптират към стресови условия в околната среда.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - ASA (Alternative Splicing in Developmental Control and Stress Response in Arabidopsis thaliana)
Alternative splicing (AS) has recently emerged as one of the most important mechanisms for generating proteome diversity and regulating gene expression. Very few examples of alternatively-spliced transcripts have been reported in plants and still fewer are known to generate functionally distinct proteins. However, recent bioinformatics studies indicate that AS plays a far more important role in plants than previously thought and further work is necessary to uncover the significance and regulation of this process. This project aimed at investigating both the biological relevance of AS in plant development and stress responses and the regulatory mechanisms of plant pre-mRNA splicing about which virtually nothing is known. Analysis of the tissue-specific expression patterns of all 19 Arabidopsis serine / arginine-rich (SR) proteins, which are established key players in mammalian AS, revealed that most display alternative transcripts and are highly expressed in flowers. In addition, the majority of these genes are induced by abiotic stress, pointing to a role for SR proteins in plant responses to the environment. Functional analysis of this protein family using reverse genetics is underway. A knockout mutant of a plant-specific SR protein displays pleiotropic phenotypic changes and altered sensitivity to sugars, indicating an essential role for this splicing factor in normal plant development and in the glucose signalling pathway. Moreover, we identified a novel E3 ubiquitin ligase that undergoes alternative splicing. Exon skipping of its pre-mRNA, determines subcellular localisation of two splice isoforms. Loss of function mutants for this gene display a subtle phenotype in the presence of ethylene, and the discovery of a highly homologous Arabidopsis gene has prompted the generation of a double mutant, which is currently being characterised. Results from this work are accelerating the understanding of the molecular mechanisms underlying plant adaptation to the environment, opening new avenues for the use of biotechnology to increase plant productivity.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Alternative splicing (AS) has recently emerged as one of the most important mechanisms for generating proteome diversity and regulating gene expression. It is becoming clear that the majority of human genes encode transcripts that undergo AS, greatly enhancing the coding potential of the genome. Very few examples of alternatively spliced transcripts have been reported in plants and still fewer are known to generate functionally distinct proteins. However, the recent explosive growth of available expressed sequence data and its alignment with genomic sequences indicates that AS plays a far more important role in plants than previously thought, and further work is necessary to uncover the significance and regulation of this process.The unique developmental plasticity and stress tolerance developed by plants as a result of their sessile growth habit suggests that they offer exceptional opportunities to reveal AS mechanisms, which are likely to play a key role in the adaptation of plants to changes in their environment. This project will investigate both the biological relevance of AS in plant development and stress responses and the regulatory mechanisms of plant pre-mRNA splicing about which virtually nothing is known.The functional significance of different splice variants of specific development- and stress-associated genes will be examined using reverse genetic approaches in Arabidopsis thaliana. Moreover, the function of individual serine/arginine-rich (SR) proteins, which are established key players in mammalian AS, and their stress-specific expression patterns will be analysed. A computational approach will also be employed to identify plant splicing enhancercis-acting elements (ESEs) recognized by SR proteins. This work deals with an exciting area of plant biology and will accelerate understanding of the molecular mechanisms underlying plant adaptation to environmental stress, opening new avenues for the use of biotechnology to increase plant productivity.
Оригинален текст от CORDIS (на английски).
Участници
- FUNDAÇÃO CALOUSTE GULBENKIAN · LISBOAКоординаторПортугалия
Връзки
Данни: CORDIS, © Европейски съюз