DCSUBTYPESPROTEOMICS · Characterisation of the functional heterogeneity of lymphoid organ dendritic cells by proteomic analysis of plasma membrane and endosomal proteins
6РП — Действия „Мария Кюри“
- Период
- 2007-10-01 → 2010-09-30
- Финансиране от ЕС
- 204 966 €
- Участници
- 2
- Схема
- OIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Дендритните клетки в лимфните органи се анализират чрез протеомика, за да се разберат разликите в разпознаването на патогени между техните подтипове. Познаването на тези функции помага за разработването на по-ефективни ваксини и терапии срещу рак, инфекции и автоимунни заболявания.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity and Management Report Summary - DCSUBTYPESPROTEOMICS (Characterisation of the functional heterogeneity of lymphoid organ dendritic cells by proteomic analysis of plasma membrane ...)
Dendritic cells provide the primary line of surveillance against pathogens and cancer cells, and initiate immune responses by activating other cells of the immune system. They are a heterogeneous population of cells and each subtype seems to play a distinct role in immune responses. The main goal of this project was to characterise the functional specialisations of the different dendritic cell types. We have analysed the whole protein composition of the 2 main dendritic cell subsets of mouse lymphoid organs using a large-scale analysis technique called proteomics. We found that many molecules were expressed differentially. We analysed in more details the functions of some of these proteins, and established that they endow dendritic cell subtypes with different abilities in terms of pathogen recognition and activation of immune responses. Understanding how dendritic cell subsets function and co-operate with each other may allow us to develop new, more effective vaccines and therapeutic treatments against cancer, autoimmune disorders and infectious diseases.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Dendritic cells (DC) are unique in their capacity to induce and regulate immune responses. Their special abilities in pathogen detection, antigen processing and presentation, and communication to T cells are determined by plasma membrane and endosomal proteins. Our knowledge of DC biology is mainly based on studies of in vitro generated-DC, which are not representative of those found in vivo. Clear evidence from the laboratories of Heath, Shortman and Villadangos show that different subtypes of DC show radical differences in antigen handling, T cell activation, and roles in immunity and tolerance.The goal of this project is to compare in depth the protein composition of different DC subtypes as they occur in vivo. We propose to use a proteomic approach to analyse plasma membrane and endosomal proteins from two major DC subtypes: CD4 DC and CD8 DC, in a resting or activated state. The most interesting molecules discovered by the proteomic analysis will be further analysed in functional studies. This project will reveal differentially expressed membrane and endosomal proteins, which could be used to target distinct DC subsets as a strategy to improve vaccination or suppress autoimmunity.The proteomic description of DC diversity we will obtain will be beneficial for the wider scientific community. The feasibility of the project is founded on the expertise and resources available to the scientists involved both at the Immunology Division, Walter and Eliza Hall Institute (Melbourne, Australia) and INSERM U653, Institut Curie (Paris, France), all of which have a demonstrated track record in their respective areas. This proposal will create the opportunity for cooperation between French and third country research institutions and allow transfer of research competencies while reinforcing the international dimension of the career of the fellow.
Оригинален текст от CORDIS (на английски).
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Данни: CORDIS, © Европейски съюз