GENETIC BARCODING · Determining the relationships between haematopoietic lineages using genetic barcoding
6РП — Действия „Мария Кюри“
- Период
- 2006-12-05 → 2008-12-04
- Финансиране от ЕС
- 150 976 €
- Участници
- 1
- Схема
- IIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Генетичният баркодинг проследява как отделните стволови клетки се превръщат в различни видове бели кръвни клетки. Това помага за по-доброто разбиране на развитието на клетките, което е важно при лечението на левкемия, лимфоми или след химиотерапия.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - GENETIC BARCODING (Determining the relationships between haematopoietic lineages using genetic barcoding)
The way in which white blood cells grow in the body, known as haematopoiesis, is a very important issue to understand so that we may better target diseases such as leukemia and lymphoma, or boost the immune system of patients after an infectious disease or chemotherapy. The current concepts state that a haematopoietic stem cell is like a seed that grows into a tree, able to generate all different branches and leaves of the white blood cell family. Most relevant studies focused on testing a large number of these cells, e.g. thousands, in mouse models. However, in order to truly understand how this process occurs, we must understand how each of those thousand cells contributes. A new technology, termed cellular barcoding was used to study this process by tagging up to 5 000 cells with a unique identifier barcode. In this way, I tracked how hundreds of single cells made the different cell types. Our preliminary data suggested that, unlike a hematopoietic stem cell (HSC) that produced many different white blood cell types, there were many different stem cell types that could sometimes make only one or two white blood cell types rather than all of them. This had important implications in how we understood development in general, and how this might be applied to therapy.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The immune system is made up of a series of white blood cell types, or leukocytes, that develop as a distinct lineage. They derive from haematopoietic stem cells (HSC), which divide and branch through several intermediate progenitor stages en route to leukocyte generation. Distortion of this process can lead to unchecked numbers as in leukaemia and lymphoma, or to deficiency of a particular immune cell. To influence the formation of distinct subsets of blood cells in the clinic, it is essential to first determine the branch points of progenitors and the degree of kinship between leukocyte progeny.The identification of clonal progenitors has been used to determine these branch points. However, their characterisation requires single progenitor cell assays, which are technically demanding. Genetic barcoding is a novel technique that allows stable integration of a unique stretch of known nucleotides (the barcode) into the DNA of progenitors, such that they are maintained and can be screened in subsequent daughter progeny.The barcodes, in effect, become the single cell tracking mechanism in this technique, thereby allowing a clonal assay but with the advantage of studying cells at a population level. This proposal aims to use genetic barcoding to assess i) the relationships of leukocytes to progenitors, ii) to determine how differentiation pathways alter upon stress, and iii) whether a true map of steady-state haematopoiesis can be mapped phylogenetically. Upon its successful application, it is envisaged that genetic barcoding can be used to address questions in development of many tissue types with potential application in stem cell therapies.This is hoped to partly encourage the competitiveness and attractiveness of European science to international scientists and investors. This proposal also aims to foster crossover of interdisciplinary skills between the European and Australian research sectors with view to creating long-standing collaborations.
Оригинален текст от CORDIS (на английски).
Участници
- VERENIGING HET NEDERLANDS KANKER INSTITUUT · AMSTERDAMКоординаторНиво градНидерландия
Връзки
Данни: CORDIS, © Европейски съюз