FLOWER P450S · Functional analysis of orphan cytochrome P450 genes in A. thaliana: P450s and flower development
6РП — Действия „Мария Кюри“
- Период
- 2007-02-01 → 2009-01-31
- Финансиране от ЕС
- 159 353 €
- Участници
- 1
- Схема
- IIF
Линиите свързват координатора с партньорите.
Накратко на български
Ензимите цитохром P450 при растението Arabidopsis thaliana определят как се създават нови метаболитни пътища за развитието на цветовете и прашеца. Анализът им помага да се разбере как чрез генетични промени растенията се адаптират и еволюират.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - FLOWER P450S (Functional analysis of orphan cytochrome P450 genes in A. thaliana: P450s and flower development)
The IBMP paper in Science (September 25 2009) describes birth of a new metabolic pathway, Metabolic plasticity, which largely relies on the creation of new genes, is an essential feature of plant adaptation and speciation and has led to evolution of large gene families. A typical example is provided by the diversification of cytochrome P450 enzymes in plants. The IBMP team «Cytochromes P450» with the support of the Marie Curie IIF programme just described the evolutionary process that led to the birth of a new metabolic pathway need for pollen development in Arabidopsis thaliana. A retroposition, neofunctionalization and duplication sequence via selective and local amino acid replacement led to the evolution of a novel phenolic pathway in Brassicaceae. This pathway involves a cascade of 6 successive hydroxylations by two partially redundant cytochromes P450 leading to the formation of N1,N5-di(hydroxyferuloyl)-N10-sinapoyl spermidine, a major pollen constituent and so far overlooked player in phenylpropanoid metabolism. This example shows how positive Darwinian selection can favour structured clusters of non-synonymous substitutions needed for transition to new function.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Reproduction is one of the most important events in an organism. In crops, it governs seed yields, viability and organoleptic properties. The regulation of reproduction happens in plants at the flowering stage.Thus understanding of the whole process, including as initiation, signalling, growth and induction of second metabolites biosynthesis during flower development is necessary to clarify the mechanism of plant reproduction. Cytochrome P450 (P450) is one of the largest enzyme families in higher plant. P 450s are involved in the biosynthesis of various secondary metabolites related to defence responses or developmental events, as key monooxygenases.In my host institution, a global P450 transcriptome analysis demonstrated that more than 120 P450 genes are expressed during flower development in Arabidopsis thaliana. Among them, five genes are expressed in the tapetum during the early stages of the pollen development. Three of these genes are activated upon insect attack. Thus their products might be involved in signalling and regulation of pollen development, or synthesis of physical barriers in higher plant.My goal is to figure out 1) what is their biochemical substrate, and 2) what is the biological role of their products in plant development and defence. This will be achieved using existing silenced and over-expressing mutants of the genes of interest for metabolic profiling, analysis of the pollen structure and biology (viability, germination), and testing for insect resistance.Completely successful work shouldestablish a correlation between P450 expression, metabolic activity, pollen composition and properties, and variable defence response. It should therefore help understanding co-evolution of development and defence.
Оригинален текст от CORDIS (на английски).
Участници
- CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · PARISКоординаторФранция
Връзки
Данни: CORDIS, © Европейски съюз