PX-NTAIL INTERACTION · Characterization of the structure and dynamics of a protein complex indispensable for RNA synthesis in Sendai Virus
6РП — Действия „Мария Кюри“
- Период
- 2005-09-01 → 2006-08-31
- Финансиране от ЕС
- 0 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Взаимодействието между два протеина при вируса Сендай се анализира чрез ядреномагнитен резонанс, за да се разбере как се синтезира неговата РНК. Това помага за по-доброто разбиране на процесите на транскрипция при семейството от вируси, които причиняват заболявания при хора и животни.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Цел на проекта
Sendai Virus (SeV) is a prototype of viruses with a negative strand RNA genome. This family of viruses causes important human and animal diseases. RNA synthesis in these viruses requires the placement of the RNA polymerase on the nucleocapsid that assemble s the RNA. This occurs through an interaction between two protein domains: the partly folded PX domain of phosphoprotein P that is part of the polymerase, and the unstructured N-tail domain of nucleocapsid protein N.For transcribing the template RNA, the polymerase needs to move along the RNA, which requires PX to on and off interact with N-tail domains on subsequent nucleocapsids. This suggests that flexibility is a key issue in the biological function of these domains. This project will study the structure and dynamics of this protein complex by NMR, in order to arrive at a better understanding of the transcription process in this family of viruses. Both PX (partly) and N-tail belong to the class of intrinsically unstructured proteins (IUPs), which encompasses around 25% of all eukaryotic proteins. NMR is one of the sole techniques that are capable of studying this class proteins and this project aims at improving NMR methodology optimised for IUPs.The project is part of an ongoing collaboration between the host lab and virus group of Prof. R. Ruigrok at the EMBL in Grenoble. This allows me to both acquire skills related to molecular biology as well as to enlarge my knowledge of NMR in order to further develop myself as a bio-molecular NMR spectroscopist. Furthermore, the experiences in NMR application and methodology acquired during my PhD will prove very valuable for the realization of the project.
Оригинален текст от CORDIS (на английски).
Участници
- INSTITUT DE BIOLOGIE STRUCTURALE JEAN-PIERRE EBELКоординаторНиво градФранция
Връзки
Данни: CORDIS, © Европейски съюз