TISSUE POLARITY · Establishment and maintenance of cell polarity and tissue architecture in the lateral-line system of the zebrafish
6РП — Действия „Мария Кюри“
- Период
- 2008-07-01 → 2010-06-30
- Финансиране от ЕС
- 146 366 €
- Участници
- 1
- Схема
- IIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Механизмите за подреждане на сензорните клетки в страничната линия на зебрата се анализират чрез сравнение между здрави риби и мутанти. Това помага да се разбере как клетките определят посоката си, за да усещат правилно движението на водата.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity and Management Report Summary - TISSUE POLARITY (Establishment and maintenance of cell polarity and tissue architecture in the lateral-line system of the zebrafish)
The zebrafish senses directional water movements with a functionally sophisticated, but anatomically simple organ called the lateral line (LL). This organ is formed by a collection of isolated units called neuromasts, each of them composed by a core of mechanosensory hair cells (HC) surrounded by supporting cells and innervated by afferent and efferent neurons. LL formation starts at 19hours-post-fertilisation (hpf), when a primordium located near the otic vesicle starts migrating along the horizontal myoseptum of the fish, depositing clusters of cells that will form the neuromasts. During migration, cells in the primordium divide, change shape and differentiate. At 40 hpf, 7 to 9 neuromasts can be observed along the body of the fish. Sensory HC in the neuromasts display a characteristic planar cell polarity (PCP) pattern, evidenced by the asymmetric localisation of the kinocilium and its associated stereocilia. The axis of morphological polarity of the stereocilia corresponds to the direction of excitability of the hair cells. Each neuromast harbours two populations of sensory HC, equal in number, whose stereocilia are oriented at 180º relative to each other. Afferent neurons innervating them can discriminate their polarity and form stable synapses with cells of identical orientation. Trilobite (tri) mutants, that lack the PCP protein Vangl2, present randomised HC polarisation in the neuromast. In this project we use transgenic lines, molecular markers and SPIM and confocal mircroscopy to investigate the mechanisms governing the establishment of HC polarisation and innervation, in wild type animals and tri mutants. We observe that early events of LL development- primordium migration, rosette formation, neuromast deposition and the axis of HC-precursor division- are not altered in these mutants. Preliminary, single-neuron analyses show that afferents contact HC, but their arborisation is more complex and synapses establishment are more dynamic than in wild type fish. In the context of this project we also screened a collection of Gal4 enhancer-trap lines and selected those driving Gal4 expression in the pLL organ of the fish. By using specific antibodies and time lapse analyses I characterised four new stable transgenic lines allowing expression of UAS elements in HC, HC progenitors, LL ganglion and lateralis glia.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The establishment and maintenance of cellular polarity are critical for organ function. In addition to the ubiquitous apicobasal axis, many epithelial cells display a second polarity axis within the plane of the tissue, referred to as planar cell polarity. The orientation of hair-cell stereocilia in the inner ear represents a striking example of planar polarity in vertebrates. Although many of the proteins involved in the interpretation of the polarising cues have been identified, the mechanistic bases of t heir activity are not known.The motivation of this project is to understand the molecular and cellular mechanisms that generate the precise architecture of a sensory epithelium, with an emphasis on planar cell polarity. Hair cells provide an excellent mod el for cell-polarity studies. Experiments will be done using the anatomically simple mechanosensory organs of the lateral line in the zebrafish, whose hair cells display a stereotyped planar polarity pattern. I will first investigate the cellular mechanism s that lead to the planar polarisation of hair cells during lateral-line development. Second, I will study how the polarised organisation is recovered after hair-cell regeneration.Preliminary evidence suggests that functional properties of progenitor cell s within the organ are determinants of hair-cell polarisation. I shall test this hypothesis by tracking protein localisation patterns during hair-cell development in vivo, in wild type and mutant specimens with randomised planar polarity. To facilitate the se analysis, I will use transgenic reporter lines with specific expression in the lateral line, in combination with fluorescently tagged proteins that distribute asymmetrically in hair cells. This innovative approach shall lead to a deeper understanding of the mechanisms governing the formation and repair of sensory organs in vertebrates, a process that I plan to explore further as an independent investigator.
Оригинален текст от CORDIS (на английски).
Участници
- CENTRE DE REGULACIÓ GENÒMICA · BARCELONAКоординаторНиво градИспания
Връзки
Данни: CORDIS, © Европейски съюз