ACDAZ · Asymmetric cell division of Arabidopsis Zygote
6РП — Действия „Мария Кюри“
- Период
- 2008-10-01 → 2010-03-31
- Финансиране от ЕС
- 148 021 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите.
Накратко на български
Асиметричното делене на зиготата при растението арабидопсис създава две различни клетки, от които се развиват стъблото и корена. Разбирането на този процес на молекулярно ниво помага да се разбере как се формира тялото на растението и как се регулира неговият растеж.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - ACDAZ (Asymmetric Cell Division of Arabidopsis Zygote)
Asymmetric cell division generates two daughter cells that adopt different developmental fates and are often different in size. This vital type of cell division occurs in a wide range of organisms, from bacteria and yeasts to plants and animals, and plays crucial roles during multicellular development. Although post-embryonic development predominates in higher plants such as arabidopsis, the basic body plan is established during embryogenesis and, during this stage, the very first asymmetric cell division is crucial for all subsequent development. The arabidopsis zygote divides asymmetrically to give an apical and a basal daughter cell. The apical daughter cell produces two tiers, each consisting of four cells, which find the apical and central regions of the embryo that give rise to the shoot meristem and hypocotyl. The basal daughter cell produces a file of six to nine cells of which all, but the uppermost one, form the suspensor. The uppermost cell of the file becomes the hypophysis, or founder cell of the basal region of the embryo from which the root meristem originates. The aim of this project was to characterise the asymmetric cell division of the zygote and the establishment of apical versus basal cell fate at the molecular level. We identified specific markers that were exclusive to the embryo and to the apical or basal lineage and were necessary to generate a high-resolution transcriptional map. In addition, we identified a negative regulator of bodenlos (BDL) expression and a positive regulator of both monopteros (MP) and BDL expression. As such, our novel and exciting results shed new light on the role of auxin in patterning and showed that auxin-independent transcriptional regulation of Aux/IAA proteins was crucial for embryonic patterning and plant growth, which represented a novel concept in plant developmental biology.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Asymmetric cell division generates two daughter cells that adopt different developmental fates and often differ in size. For example, a stem cell generates another stem cell and a daughter cell that gives rise to differentiated cells. This vital type of cell division occurs in a wide range of organisms, from bacteria and yeasts to plants and animals, and plays crucial roles during multicellular development. Molecular mechanisms of asymmetric cell division have been studied in animal model systems, such as Drosophila and C.elegans but barely addressed in plants. However, mechanistic differences are to be expected between the two groups since plants and animals have evolved independently. Although post-embryonic development predominates in higher plants such as Arabidopsis, the basic body plan is established during embryogenesis and here, the very first asymmetric cell division is crucial for all subsequent development. The Arabidopsis zygote divides asymmetrically to give an apical and a basal daughter cell. These two cells differ in gene expression, cell size and cell division program, and give rise to different pattern elements of the embryo. Gene expression studies indicate that already at the earliest stages of embryogenesis, specific transcription programs are initiated in single precursor cells of embryo pattern elements. Furthermore, recent findings on the directional movement of the plant signalling molecule auxin provide a unique association between individual cell polarity and the establishment of polarity at the tissue, organ and whole plant level. The aim of this project is to characterize the asymmetric cell division of the zygote and the establishment of apical versus basal cell fate at the molecular level. Our objectives are to: (1) generate and analyze fluorescent molecular markers, (2) search for regulators of BDL expression, and (3) identify transcriptional profiles of apical and basal cell.
Оригинален текст от CORDIS (на английски).
Участници
- EBERHARD KARLS-UNIVERSITAET TUEBINGEN · TUEBINGENКоординаторГермания
Връзки
Данни: CORDIS, © Европейски съюз