DYNAMIC MICRODOMAINS · Dynamics of DC-SIGN microdomains mediating recognition and uptake of viruses by dendritic cells
6РП — Действия „Мария Кюри“
- Период
- 2005-09-01 → 2006-08-31
- Финансиране от ЕС
- 0 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Дендритните клетки разпознават вируси като Ебола и ХИВ чрез специални зони на мембраната си, наречени микродомени. Разбирането на динамиката им помага при разработването на нови ваксини и терапии срещу опасни патогени.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Цел на проекта
Dendritic cells (DCs) are the sentinels of the immune system. They are bone marrow-derived, phagocytic cells, located in tissues to patrol for foreign antigens and present them to T cells. DC-SIGN is a DC-specific C-type lectin involved in adhesion and in pathogen-recognition, interacting with carbohydrate structures in a Ca2+-dependent manner. Life-threatening viruses like HIV-1, Ebola, and Hepatitis C virus are recognized by DC-SIGN. By high-resolution electron microscopy (EM), the applicant showed that o n DC cell membrane DC-SIGN can exist in two forms: randomly distributed or in submicron-sized (200 nm diameter) microdomains. These microdomains were shown to reside in lipid rafts and to be required for efficient binding and uptake of virus. How DC-SIGN m icrodomains are formed is largely unknown. The aim of this proposal is to follow the dynamics of DC-SIGN microdomains in living cells with high spatial and temporal resolution. Two aspects will be investigated: formation and stability of DC-SIGN microdomai ns as well as microdomain-mediated internalization and routing of virus-like particles. Fluorescence Resonance Energy Transfer (FRET) is a powerful tool for imaging molecular activities of suitably labeled DC-SIGN molecules in living cells. Ligand-coated h ighly-photostable Quantum Dots are excellent fluorescent probes for monitoring endocytosis and vesicular trafficking mediated by DC-SIGN micro-domains. Currently, DCs are manipulated ex vivo for the development of anti-tumour and anti-virus therapies. Therefore, this project will provide relevant information for important health-care areas, such asi) development of C-type lectin-based vaccines against invading pathogens; ii) study of antigen-routing mechanisms in DC cross-presentation; and iii) development o f C-type lectin-based targeting of DCs in anti-tumour strategies.
Оригинален текст от CORDIS (на английски).
Участници
- MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRYКоординаторНиво градГермания
Връзки
Данни: CORDIS, © Европейски съюз