VIRUS ENTRY · Cell entry of influenza a virus and human cytomegalovirus
6РП — Действия „Мария Кюри“
- Период
- 2006-12-01 → 2007-06-30
- Финансиране от ЕС
- 173 831 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.
Накратко на български
Механизмите, по които грипният вирус прониква в човешките клетки, се анализират чрез идентифициране на необходимите за това протеини и гени. Това помага за разработването на по-устойчиви антивирусни лекарства, тъй като вирусите често мутират и стават устойчиви на съществуващите терапии.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - VIRUS ENTRY (Cell entry of influenza a virus and human cytomegalovirus)
Influenza virus belongs to the class of emerging pathogens and due to recent threats of new pandemics attracts even more public attention. As most viruses influenza viruses have the ability to mutate rapidly emerging thereby creating new strains with unknown human pathogenic potential. Numerous attempts were made by industry to develop an antiviral drug against Influenza which keeps functional over a longer period of time. The last example Tamiflu already showed escape mutants recently. Therefore it is even of more relevance to investigate the injection mechanism of Influenza virus in more detail. After binding of the virus to the cell surface the virus needs to be endocytosed in order to enter the cell and fuse at a later step with host cell membrane. This research project was centred on the fundamental question how influenza virus enters the cell in general and to identify essential factors required. First of all two independent ways how to enter the cell could be identified for influenza virus and characterised in more molecular detail. In order to identify host cell factors required for influenza viruses endocytosis a new screening system was established to investigate the role of host cell factors in this process systematically which in principle allows studying the role of all human genes in the infection mechanism of influenza viruses.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Outbreaks of influenza A in humans, birds and animals constitute a continuous major threat to public health as influenza A viruses are capable of species shifts and enormous genetic variation. More than two decades ago, influenza entry was described to occur by endocytosis via clathrin-coated pits. Some evidence was also found for an alternative, clathrin-independent endocytic pathway, but it has not received attention until recently. I will focus on the novel, alternative process. Another virus becoming clinically more important, human cytomegalovirus (hCMV), can be detected in over 80% of the worlds population. Although most humans keep a latent infection without any symptoms, the virus can be reactivated in immuno-compromised individuals. Furthermore, severe mental defects as well as deafness can occur in case of congenital infection of newborns which is up to 1% of all newborns in the USA.The endocytic entry mechanism of hCMV is largely unknown. Only recently specific integrin-subunits were identified as receptors. I propose to investigate the entry mechanism of hCMV in more detail. In general, the whole analysis will be carried out in a comparative manner to two already quite well studied model pathogens, namely simian virus 40 (SV40) and semliki forest virus (SFV). Experimentally, I will investigate the behaviour of individual, labelled viruses in and on cells using state of the art life-cell-imaging microscopy. I will set up screening assays for influenza A and hCMV infection applying siRNAs in a loss of function screen. Construction of stable cell lines harbouring a conditional RNAi / shRNA knockdown system targeting the clathrin-dependent uptake mechanism will enable me to separate the pathway from the parallel clathrin-dependent pathway.These result s are expected to provide crucial information on endocytosis in general as well as reveal key steps in the entry process offering targets for antiviral therapy based on cellular factors.
Оригинален текст от CORDIS (на английски).
Участници
- EIDGENOSSISCHE TECHNISCHE HOCHSCHULE ZURICH · ZURICHКоординаторНиво градШвейцария
Връзки
Данни: CORDIS, © Европейски съюз